@article{da7f50014c80430ca54b7f12f6b813f7,
title = "Identification of bullous pemphigoid, pemphigus, laminin, and anchoring fibril antigens in human fetal skin",
abstract = "Human fetal skin was evaluated for sequential and regional development of several epidermal antigens. Indirect immunofluorescent methods were used to detect laminin, bullous pemphigoid antigen, pemphigus antigen, and anchoring fibril antigens identified by monoclonal antibodies AF1 and AF2. Eighty-three human fetal skin biopsies from 32 human fetuses were examined. The fetuses examined ranged from estimated gestational age (EGA) of 7-38 weeks. Laminin was present in the basement membrane zone of all the fetal tissues examined. Bullous pemphigoid antigen developed first in the palm and sole, 9 weeks EGA, and was present in all other sites by 17 weeks EGA. Pemphigus antigen was present by 11 weeks EGA. AF1 and AF2 staining was not present until 26 weeks EGA. AF1 and AF2 stained epidermal basal cells in addition to the basement membrane zone area. Comparison of human fetal skin development with basal cell carcinoma identified similarities between basal cell carcinoma and early fetal development.",
author = "Lane, {A. T.} and Helm, {K. F.} and Goldsmith, {L. A.}",
note = "Funding Information: Eighty-three human fetal skin biopsies, from 32 human fetuses, were examined. Human fetal tissue collection conformed to current recommendations of t;he NIH and recommendations of the University of Rochester Committee on Investigation Involving Human Subjects. Signed informed consent was obtained. The fetuses examined ranged from estimated gestational age (EGA) of 7-38 weeks. Fetal tissues under 24 weeks EGA were the products of conception of human abortions. Fetal skin biopsies over 24 weeks EGA were removed from premature infants who had died within 24 h of birth. The ge~tational age of the infant was established by the EGA taken from the maternal history correlated with the heel-toe length standards (4] when possible. The abortion tissue specimens were collected within 2 h after extraction from the human uterus. The tissue sections were frozen in OCT (LabTek Products, Naperville, lllinois) and stored at - 70{"}C until immu- Manuscript received Apri l 6, 1984; accepted for publication June 25, 1984. This work was supported by National Institutes of Health grants 1 K08 AM01212 (ATL), 5 ROl AM30126 (LAG), 2 ROl AM30965 (LAG) , and HHS grant HL-07496 from the NIH (KFH). Reprint requests to: Alfred T. Lane, M.D., Dermatology Unit, P.O. Box 697, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, New York 14642. Abbreviations: EGA: estimated gestational age PBS: phosphate-buffered saline",
year = "1985",
doi = "10.1111/1523-1747.ep12274612",
language = "English (US)",
volume = "84",
pages = "27--30",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Elsevier B.V.",
number = "1",
}