TY - JOUR
T1 - Identification of ellagic acid rhamnoside as a bioactive component of a complex botanical extract with anti-biofilm activity
AU - Fontaine, Benjamin M.
AU - Nelson, Kate
AU - Lyles, James T.
AU - Jariwala, Parth B.
AU - García-Rodriguez, Jennifer M.
AU - Quave, Cassandra L.
AU - Weinert, Emily E.
N1 - Publisher Copyright:
© 2017 Fontaine, Nelson, Lyles, Jariwala, García-Rodriguez, Quave and Weinert.
PY - 2017/3/23
Y1 - 2017/3/23
N2 - Staphylococcus aureus is a leading cause of hospital-acquired infections. It is listed among the top "serious threats" to human health in the USA, due in large part to rising rates of resistance. Many S. aureus infections are recalcitrant to antibiotic therapy due to their ability to form a biofilm, which acts not only as a physical barrier to antibiotics and the immune system, but results in differences in metabolism that further restricts antibiotic efficacy. Development of a modular strategy to synthesize a library of phenolic glycosides allowed for bioactivity testing and identification of anti-biofilm compounds within an extract of the elmleaf blackberry (Rubus ulmifolius). Two ellagic acid (EA) derivatives, EA xyloside and EA rhamnoside, have been identified as components of the Rubus extract. In addition, EA rhamnoside has been identified as an inhibitor of biofilm formation, with activity comparable to the complex extract 220D-F2 (composed of a mixture of EA glycosides), and confirmed by confocal laser scanning microscopy analyses.
AB - Staphylococcus aureus is a leading cause of hospital-acquired infections. It is listed among the top "serious threats" to human health in the USA, due in large part to rising rates of resistance. Many S. aureus infections are recalcitrant to antibiotic therapy due to their ability to form a biofilm, which acts not only as a physical barrier to antibiotics and the immune system, but results in differences in metabolism that further restricts antibiotic efficacy. Development of a modular strategy to synthesize a library of phenolic glycosides allowed for bioactivity testing and identification of anti-biofilm compounds within an extract of the elmleaf blackberry (Rubus ulmifolius). Two ellagic acid (EA) derivatives, EA xyloside and EA rhamnoside, have been identified as components of the Rubus extract. In addition, EA rhamnoside has been identified as an inhibitor of biofilm formation, with activity comparable to the complex extract 220D-F2 (composed of a mixture of EA glycosides), and confirmed by confocal laser scanning microscopy analyses.
UR - http://www.scopus.com/inward/record.url?scp=85016555730&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016555730&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2017.00496
DO - 10.3389/fmicb.2017.00496
M3 - Article
AN - SCOPUS:85016555730
SN - 1664-302X
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - MAR
M1 - 496
ER -