Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

Richard J. Wang; B. Tracy Nixon

doi:10.1007/BF02616168

Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

Richard J. Wang, B. Tracy Nixon

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to “daylight” fluorescent light. Addition of pure H₂O₂ and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H₂O₂ during light exposure.

Original language	English (US)
Pages (from-to)	715-722
Number of pages	8
Journal	In Vitro
Volume	14
Issue number	8
DOIs	https://doi.org/10.1007/BF02616168
State	Published - Aug 1978

All Science Journal Classification (ASJC) codes

Biotechnology
Plant Science

Access to Document

10.1007/BF02616168

Cite this

@article{ec83c6abc52f4eda9ae017345995539e,

title = "Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light",

abstract = "Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to “daylight” fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.",

author = "Wang, {Richard J.} and Nixon, {B. Tracy}",

year = "1978",

month = aug,

doi = "10.1007/BF02616168",

language = "English (US)",

volume = "14",

pages = "715--722",

journal = "In Vitro",

issn = "0073-5655",

publisher = "Tissue Culture Association",

number = "8",

}

TY - JOUR

T1 - Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

AU - Wang, Richard J.

AU - Nixon, B. Tracy

PY - 1978/8

Y1 - 1978/8

N2 - Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to “daylight” fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.

AB - Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to “daylight” fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.

UR - http://www.scopus.com/inward/record.url?scp=0018146110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018146110&partnerID=8YFLogxK

U2 - 10.1007/BF02616168

DO - 10.1007/BF02616168

M3 - Article

C2 - 689707

AN - SCOPUS:0018146110

SN - 0073-5655

VL - 14

SP - 715

EP - 722

JO - In Vitro

JF - In Vitro

IS - 8

ER -

Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this