TY - JOUR
T1 - Identification of ring oxidized metabolites of 1-nitropyrene in the feces and urine of germfree f344 rats
AU - El-Bayoumy, Karam
AU - Reddy, Bandaru
AU - Hecht, Stephen S.
N1 - Funding Information:
A Study of Chemical Carrinogenesis, 73. This research was supported by NCI Grant CA-35519. We wish to thank Mr Yves M. Louis for handling the germfree animals, and Mrs Lori DeMarco for typing this manuscript.
PY - 1984/10
Y1 - 1984/10
N2 - 1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.
AB - 1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.
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U2 - 10.1093/carcin/5.10.1371
DO - 10.1093/carcin/5.10.1371
M3 - Article
C2 - 6488459
AN - SCOPUS:0021188119
SN - 0143-3334
VL - 5
SP - 1371
EP - 1373
JO - Carcinogenesis
JF - Carcinogenesis
IS - 10
ER -
