TY - JOUR
T1 - Identification of sensilla involved in taste mediation in adult western corn rootworm (Diabrotica virgifera virgifera LeConte)
AU - Chyb, Sylwester
AU - Eichenseer, Herbert
AU - Hollister, Benedict
AU - Mullin, Christopher A.
AU - Frazier, James L.
PY - 1995/3/1
Y1 - 1995/3/1
N2 - A group of sensilla present on the maxillary galea of adult western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) beetles has been identified morphologically and physiologically to be involved in taste mediation. There are approximately 15 chemosensory hairs on each galea. Bilateral removal of these structures resulted in a significantly reduced consumption of a strongly phagostimulant triterpenoid, cucurbitacin B, and led to increased ingestion of a phagodeterrent alkaloid, strychnine. Electrophysiological responses obtained via tip-recording of galeal chemosensilla with submillimolar concentrations of host and nonhost plant compounds resulted in dose responses overlapping with the effective behavioral ranges. Cucurbitacin B was found to evoke chemosensory responses at levels as low as 0.1 μM. Since γ-aminobutyric acid (GABA) is an agonist. (-)-β-hydrastine and strychnine are antagonists, and cucurbitacin B has been proposed to act at a separate modulatory site of classical synaptic GABA and glycine receptor-channel complexes, results reported here raise the possibility that there are peripheral chemosensory receptor sites that may resemble, functionally and structurally, synaptic receptor sites in the central nervous system.
AB - A group of sensilla present on the maxillary galea of adult western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) beetles has been identified morphologically and physiologically to be involved in taste mediation. There are approximately 15 chemosensory hairs on each galea. Bilateral removal of these structures resulted in a significantly reduced consumption of a strongly phagostimulant triterpenoid, cucurbitacin B, and led to increased ingestion of a phagodeterrent alkaloid, strychnine. Electrophysiological responses obtained via tip-recording of galeal chemosensilla with submillimolar concentrations of host and nonhost plant compounds resulted in dose responses overlapping with the effective behavioral ranges. Cucurbitacin B was found to evoke chemosensory responses at levels as low as 0.1 μM. Since γ-aminobutyric acid (GABA) is an agonist. (-)-β-hydrastine and strychnine are antagonists, and cucurbitacin B has been proposed to act at a separate modulatory site of classical synaptic GABA and glycine receptor-channel complexes, results reported here raise the possibility that there are peripheral chemosensory receptor sites that may resemble, functionally and structurally, synaptic receptor sites in the central nervous system.
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U2 - 10.1007/BF02036720
DO - 10.1007/BF02036720
M3 - Article
AN - SCOPUS:0029139820
SN - 0098-0331
VL - 21
SP - 313
EP - 329
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
IS - 3
ER -