TY - JOUR
T1 - Identification of ubiquitin-modified lysine residues and novel phosphorylation sites on eukaryotic initiation factor 2B epsilon
AU - Tuckow, Alexander P.
AU - Kazi, Abid A.
AU - Kimball, Scot R.
AU - Jefferson, Leonard S.
N1 - Funding Information:
The authors are grateful to Holly A. Lacko for her expert technical assistance. The studies described here were supported by National Institutes of Health [Grants DK-15658 and DK-13499 (to L. S. Jefferson)] and a grant from The Pennsylvania Department of Health using Tobacco Settlement Funds. The Pennsylvania Department of Health specifically disclaims responsibility for any analyses, interpretations, or conclusions.
PY - 2013/6/21
Y1 - 2013/6/21
N2 - Eukaryotic initiation factor 2Bε (eIF2Bε) plays a critical role in the initiation of mRNA translation and its expression and guanine nucleotide exchange activity are major determinants of the rate of protein synthesis. In this work we provide evidence that the catalytic epsilon subunit of eIF2B is subject to ubiquitination and proteasome-mediated degradation. Lysates of C2C12 myoblasts treated with proteasome inhibitor were subjected to sequential immunoprecipitations for eIF2Bε followed by ubiquitin. Tandem mass spectrometry (LC-MS/MS) analysis of immunoprecipitated proteins resulted in the identification of five peptides containing ubiquitin (diglycine) modifications on eIF2Bε. The specific lysine residues containing the ubiquitin modifications were localized as Lys-56, Lys-98, Lys-136, Lys-212 and Lys-500 (corresponding to the rat protein sequence). In addition three novel phosphorylation sites were identified including Ser-22, Ser-125, and Thr-317. Moreover, peptides corresponding to the amino acid sequence of the E3 ligase NEDD4 were also detected in the LC-MS/MS analysis, and an interaction between endogenous eIF2Bε with NEDD4 was confirmed by co-immunoprecipitation.
AB - Eukaryotic initiation factor 2Bε (eIF2Bε) plays a critical role in the initiation of mRNA translation and its expression and guanine nucleotide exchange activity are major determinants of the rate of protein synthesis. In this work we provide evidence that the catalytic epsilon subunit of eIF2B is subject to ubiquitination and proteasome-mediated degradation. Lysates of C2C12 myoblasts treated with proteasome inhibitor were subjected to sequential immunoprecipitations for eIF2Bε followed by ubiquitin. Tandem mass spectrometry (LC-MS/MS) analysis of immunoprecipitated proteins resulted in the identification of five peptides containing ubiquitin (diglycine) modifications on eIF2Bε. The specific lysine residues containing the ubiquitin modifications were localized as Lys-56, Lys-98, Lys-136, Lys-212 and Lys-500 (corresponding to the rat protein sequence). In addition three novel phosphorylation sites were identified including Ser-22, Ser-125, and Thr-317. Moreover, peptides corresponding to the amino acid sequence of the E3 ligase NEDD4 were also detected in the LC-MS/MS analysis, and an interaction between endogenous eIF2Bε with NEDD4 was confirmed by co-immunoprecipitation.
UR - http://www.scopus.com/inward/record.url?scp=84879152762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879152762&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2013.05.053
DO - 10.1016/j.bbrc.2013.05.053
M3 - Article
C2 - 23707720
AN - SCOPUS:84879152762
SN - 0006-291X
VL - 436
SP - 41
EP - 46
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -