IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

M. Manocha; M. S. Shajib; M. M. Rahman; H. Wang; P. Rengasamy; M. Bogunovic; M. Jordana; L. Mayer; W. I. Khan

doi:10.1038/mi.2012.58

IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

M. Manocha, M. S. Shajib, M. M. Rahman, H. Wang, P. Rengasamy, M. Bogunovic, M. Jordana, L. Mayer, W. I. Khan

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Abstract

Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

Original language	English (US)
Pages (from-to)	146-155
Number of pages	10
Journal	Mucosal Immunology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/mi.2012.58
State	Published - Jan 2013

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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10.1038/mi.2012.58

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title = "IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut",

abstract = "Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.",

author = "M. Manocha and Shajib, {M. S.} and Rahman, {M. M.} and H. Wang and P. Rengasamy and M. Bogunovic and M. Jordana and L. Mayer and Khan, {W. I.}",

note = "Funding Information: This work is supported by the grants from the Canadian Institutes of Health Research (CIHR) and Crohn {\textquoteright}s and Colitis Foundation of Canada (CCFC) to Dr Khan. Dr Khan is a recipient of CIHR New Investigator Award. The authors thank Dr SM Atiqur Rahman for his valuable input.",

year = "2013",

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AU - Manocha, M.

AU - Shajib, M. S.

AU - Rahman, M. M.

AU - Wang, H.

AU - Rengasamy, P.

AU - Bogunovic, M.

AU - Jordana, M.

AU - Mayer, L.

AU - Khan, W. I.

N1 - Funding Information: This work is supported by the grants from the Canadian Institutes of Health Research (CIHR) and Crohn ’s and Colitis Foundation of Canada (CCFC) to Dr Khan. Dr Khan is a recipient of CIHR New Investigator Award. The authors thank Dr SM Atiqur Rahman for his valuable input.

PY - 2013/1

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N2 - Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

AB - Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

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IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

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