IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

M. Manocha; M. S. Shajib; M. M. Rahman; H. Wang; P. Rengasamy; M. Bogunovic; M. Jordana; L. Mayer; W. I. Khan

doi:10.1038/mi.2012.58

IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

M. Manocha
, M. S. Shajib
, M. M. Rahman
, H. Wang
, P. Rengasamy
, M. Bogunovic
, M. Jordana
, L. Mayer
, W. I. Khan

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

Original language	English (US)
Pages (from-to)	146-155
Number of pages	10
Journal	Mucosal Immunology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/mi.2012.58
State	Published - Jan 2013

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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@article{f385a28b41764e2791ccae1a1b5353fc,

title = "IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut",

abstract = "Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.",

author = "M. Manocha and Shajib, \{M. S.\} and Rahman, \{M. M.\} and H. Wang and P. Rengasamy and M. Bogunovic and M. Jordana and L. Mayer and Khan, \{W. I.\}",

note = "Funding Information: This work is supported by the grants from the Canadian Institutes of Health Research (CIHR) and Crohn {\textquoteright}s and Colitis Foundation of Canada (CCFC) to Dr Khan. Dr Khan is a recipient of CIHR New Investigator Award. The authors thank Dr SM Atiqur Rahman for his valuable input.",

year = "2013",

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doi = "10.1038/mi.2012.58",

language = "English (US)",

volume = "6",

pages = "146--155",

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AU - Manocha, M.

AU - Shajib, M. S.

AU - Rahman, M. M.

AU - Wang, H.

AU - Rengasamy, P.

AU - Bogunovic, M.

AU - Jordana, M.

AU - Mayer, L.

AU - Khan, W. I.

N1 - Funding Information: This work is supported by the grants from the Canadian Institutes of Health Research (CIHR) and Crohn ’s and Colitis Foundation of Canada (CCFC) to Dr Khan. Dr Khan is a recipient of CIHR New Investigator Award. The authors thank Dr SM Atiqur Rahman for his valuable input.

PY - 2013/1

Y1 - 2013/1

N2 - Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

AB - Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.

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IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut

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