IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

Marion Scharte; Kerstin Jurk; Beate Kehrel; Alexander Zarbock; Hugo Van Aken; Kai Singbartl

doi:10.1016/j.febslet.2006.10.053

IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

Marion Scharte, Kerstin Jurk, Beate Kehrel, Alexander Zarbock, Hugo Van Aken, Kai Singbartl

Department of Anesthesiology and Perioperative Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O₂) and hypoxia (1% O₂). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.

Original language	English (US)
Pages (from-to)	6399-6404
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2006.10.053
State	Published - Nov 27 2006

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.10.053

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title = "IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells",

abstract = "Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O2) and hypoxia (1% O2). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.",

author = "Marion Scharte and Kerstin Jurk and Beate Kehrel and Alexander Zarbock and Aken, {Hugo Van} and Kai Singbartl",

note = "Funding Information: This study was supported by the Ministerium f{\"u}r Wissenschaft und Forschung of the county NRW/Germany Lise-Meitner Grant 134.1.06.01.05/04 and the University of Muenster/Germany IMF Grant SC 220402.",

year = "2006",

month = nov,

day = "27",

doi = "10.1016/j.febslet.2006.10.053",

language = "English (US)",

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pages = "6399--6404",

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T1 - IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

AU - Scharte, Marion

AU - Jurk, Kerstin

AU - Kehrel, Beate

AU - Zarbock, Alexander

AU - Aken, Hugo Van

AU - Singbartl, Kai

N1 - Funding Information: This study was supported by the Ministerium für Wissenschaft und Forschung of the county NRW/Germany Lise-Meitner Grant 134.1.06.01.05/04 and the University of Muenster/Germany IMF Grant SC 220402.

PY - 2006/11/27

Y1 - 2006/11/27

N2 - Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O2) and hypoxia (1% O2). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.

AB - Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O2) and hypoxia (1% O2). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.

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ER -

IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

Abstract

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