Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

M. K. Hong; A. G. Jeung; N. V. Dokholyan; T. I. Smith; H. A. Schwettman; P. Huie; S. Erramilli

doi:10.1016/S0168-583X(98)00311-5

Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

M. K. Hong, A. G. Jeung, N. V. Dokholyan, T. I. Smith, H. A. Schwettman, P. Huie, S. Erramilli

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.

Original language	English (US)
Pages (from-to)	246-255
Number of pages	10
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	144
Issue number	1-4
DOIs	https://doi.org/10.1016/S0168-583X(98)00311-5
State	Published - Sep 2 1998

All Science Journal Classification (ASJC) codes

Nuclear and High Energy Physics
Instrumentation

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10.1016/S0168-583X(98)00311-5

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Hong, M. K., Jeung, A. G., Dokholyan, N. V., Smith, T. I., Schwettman, H. A., Huie, P., & Erramilli, S. (1998). Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 144(1-4), 246-255. https://doi.org/10.1016/S0168-583X(98)00311-5

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title = "Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser",

abstract = "We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.",

author = "Hong, {M. K.} and Jeung, {A. G.} and Dokholyan, {N. V.} and Smith, {T. I.} and Schwettman, {H. A.} and P. Huie and S. Erramilli",

note = "Funding Information: Work supported in part by the National Science Foundation (DMR) and the Office of Naval Research (Contract No. N00014-94-1-1024). NVD acknowledges support from the NSF (CH 9728854) and the National Institutes for Health (HG00989). S.E. acknowledges the receipt of a duPont Young Professor award. We thank S.J. Hong for advice on image analysis; D. Palanker for advice and help in AFM and fluorescence microscopy; Y.N. Jun, D. Fung, C. Smith, E. Evans for discussions; and the FEL center staff for their support and encouragement.",

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Hong, MK, Jeung, AG, Dokholyan, NV, Smith, TI, Schwettman, HA, Huie, P & Erramilli, S 1998, 'Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 144, no. 1-4, pp. 246-255. https://doi.org/10.1016/S0168-583X(98)00311-5

Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser. / Hong, M. K.; Jeung, A. G.; Dokholyan, N. V. et al.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 144, No. 1-4, 02.09.1998, p. 246-255.

Research output: Contribution to journal › Article › peer-review

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T1 - Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

AU - Hong, M. K.

AU - Jeung, A. G.

AU - Dokholyan, N. V.

AU - Smith, T. I.

AU - Schwettman, H. A.

AU - Huie, P.

AU - Erramilli, S.

N1 - Funding Information: Work supported in part by the National Science Foundation (DMR) and the Office of Naval Research (Contract No. N00014-94-1-1024). NVD acknowledges support from the NSF (CH 9728854) and the National Institutes for Health (HG00989). S.E. acknowledges the receipt of a duPont Young Professor award. We thank S.J. Hong for advice on image analysis; D. Palanker for advice and help in AFM and fluorescence microscopy; Y.N. Jun, D. Fung, C. Smith, E. Evans for discussions; and the FEL center staff for their support and encouragement.

PY - 1998/9/2

Y1 - 1998/9/2

N2 - We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.

AB - We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.

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IS - 1-4

ER -

Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

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