TY - GEN
T1 - Immobilization of chondroitin sulfate for the fabrication of biomimetic brush structures
AU - Sarkar, S.
AU - Ganesh, N.
AU - Schauer, C. L.
AU - Vresilovic, E.
AU - Marcolongo, M.
PY - 2010
Y1 - 2010
N2 - Intervertebral disc (IVD) degeneration is accompanied by a loss of proteoglycans (PGs) in the nucleus pulposus (NP), resulting in a loss of charged chondroitin sulfate (CS) chains, decreasing mechanical function of the disc. PG mimics may be employed to restore NP functionality. Synthesis of CS bottle brush structures requires immobilization of CS at its terminal end. In this study, we have investigated commercially available CS for use in CS bottle brush synthesis. A primary amine (PA) at the terminal end of CS was identified and utilized to conjugate amine-reactive monomers to CS. Conjugation of monomers to CS was confirmed using the fluorescamine assay. Percent PA conjugations of 26%, 80% and 99% were seen for acrolein, acrylic acid and allyl glycidyl ether respectively. CS was also immobilized onto epoxy and aldehyde functionalized surfaces via the CS terminal primary amine. CS attachment was determined by monitoring changes in substrate sulfur content (energy-dispersive X-ray EDAX microanalysis) and surface wettability (contact angle). The modification and immobilization of CS demonstrated here will facilitate the synthesis of CS bottle brush PG mimics.
AB - Intervertebral disc (IVD) degeneration is accompanied by a loss of proteoglycans (PGs) in the nucleus pulposus (NP), resulting in a loss of charged chondroitin sulfate (CS) chains, decreasing mechanical function of the disc. PG mimics may be employed to restore NP functionality. Synthesis of CS bottle brush structures requires immobilization of CS at its terminal end. In this study, we have investigated commercially available CS for use in CS bottle brush synthesis. A primary amine (PA) at the terminal end of CS was identified and utilized to conjugate amine-reactive monomers to CS. Conjugation of monomers to CS was confirmed using the fluorescamine assay. Percent PA conjugations of 26%, 80% and 99% were seen for acrolein, acrylic acid and allyl glycidyl ether respectively. CS was also immobilized onto epoxy and aldehyde functionalized surfaces via the CS terminal primary amine. CS attachment was determined by monitoring changes in substrate sulfur content (energy-dispersive X-ray EDAX microanalysis) and surface wettability (contact angle). The modification and immobilization of CS demonstrated here will facilitate the synthesis of CS bottle brush PG mimics.
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U2 - 10.1109/NEBC.2010.5458166
DO - 10.1109/NEBC.2010.5458166
M3 - Conference contribution
AN - SCOPUS:77953073361
SN - 9781424468799
T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010
Y2 - 26 March 2010 through 28 March 2010
ER -