Immunogenetic analysis of H-2 mutations - VI. Crossreactivity in cell-mediated lympholysis between TNP-modified cells from H-2 mutant strains

Spleen cells carrying the H-2K^b allele and sensitized against TNP-modified stimulator cells in vitro displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in the H-2K^b allele (haplotypes H-2^ba, H-2^bd, and H-2^bf). Similar crossreactivity in TNP-CML was observed in the reciprocal direction. Spleen cells carrying the H-2K^k allele and sensitized against TNP-modified stimulators displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in the H-2K^k allele (haplotype H-2^ka) and vice versa. The effector cells in these assays were sensitive to anti-T cell serum in the presence of complement, and supernatants from immune cultures did not induce nonimmune cells to display a cytotoxic effect. Titration of effector cells from mutant and wild-type strains of the H-2^b haplotype indicated no detectable quantitative differences in their activities. These data demonstrate that crossreactivity in TNP-CML occurs in closely related allogeneic strains that have recently undergone mutation in the H-2 complex.