TY - JOUR
T1 - Immunological Analyses of Whole Blood via “Microfluidic Drifting” Based Flow Cytometric Chip
AU - Nawaz, Ahmad Ahsan
AU - Nissly, Ruth Helmus
AU - Li, Peng
AU - Chen, Yuchao
AU - Guo, Feng
AU - Li, Sixing
AU - Shariff, Yasir M.
AU - Qureshi, Arooj Nawaz
AU - Wang, Lin
AU - Huang, Tony Jun
N1 - Publisher Copyright:
© 2014, Biomedical Engineering Society.
PY - 2014/11
Y1 - 2014/11
N2 - Cost-effective, high-performance diagnostic instruments are vital to providing the society with accessible, affordable, and high-quality healthcare. Here we present an integrated, “microfluidic drifting” based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. It is capable of analyzing human blood for cell counting and diagnosis of diseases. Our device achieves a throughput of ~3754 events/s. Calibration with Flow-Check calibration beads indicated good congruency with a commercially available benchtop flow cytometer. Moreover, subjection to a stringent 8-peak rainbow calibration particle test demonstrated its ability to perform high-resolution immunological studies with separation resolution of 4.28 between the two dimmest fluorescent populations. Counting accuracy at different polystyrene bead concentrations showed strong correlation (r = 0.9991) with hemocytometer results. Finally, reliable quantification of CD4+ cells in healthy human blood via staining with monoclonal antibodies was demonstrated. These results demonstrate the potential of our microfluidic flow cytometry chip as an inexpensive yet high-performance point-of-care device for mobile medicine.
AB - Cost-effective, high-performance diagnostic instruments are vital to providing the society with accessible, affordable, and high-quality healthcare. Here we present an integrated, “microfluidic drifting” based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. It is capable of analyzing human blood for cell counting and diagnosis of diseases. Our device achieves a throughput of ~3754 events/s. Calibration with Flow-Check calibration beads indicated good congruency with a commercially available benchtop flow cytometer. Moreover, subjection to a stringent 8-peak rainbow calibration particle test demonstrated its ability to perform high-resolution immunological studies with separation resolution of 4.28 between the two dimmest fluorescent populations. Counting accuracy at different polystyrene bead concentrations showed strong correlation (r = 0.9991) with hemocytometer results. Finally, reliable quantification of CD4+ cells in healthy human blood via staining with monoclonal antibodies was demonstrated. These results demonstrate the potential of our microfluidic flow cytometry chip as an inexpensive yet high-performance point-of-care device for mobile medicine.
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U2 - 10.1007/s10439-014-1041-5
DO - 10.1007/s10439-014-1041-5
M3 - Article
C2 - 24893596
AN - SCOPUS:84919877185
SN - 0090-6964
VL - 42
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 11
ER -
