Immunological Analyses of Whole Blood via “Microfluidic Drifting” Based Flow Cytometric Chip

Ahmad Ahsan Nawaz, Ruth Helmus Nissly, Peng Li, Yuchao Chen, Feng Guo, Sixing Li, Yasir M. Shariff, Arooj Nawaz Qureshi, Lin Wang, Tony Jun Huang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Cost-effective, high-performance diagnostic instruments are vital to providing the society with accessible, affordable, and high-quality healthcare. Here we present an integrated, “microfluidic drifting” based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. It is capable of analyzing human blood for cell counting and diagnosis of diseases. Our device achieves a throughput of ~3754 events/s. Calibration with Flow-Check calibration beads indicated good congruency with a commercially available benchtop flow cytometer. Moreover, subjection to a stringent 8-peak rainbow calibration particle test demonstrated its ability to perform high-resolution immunological studies with separation resolution of 4.28 between the two dimmest fluorescent populations. Counting accuracy at different polystyrene bead concentrations showed strong correlation (r = 0.9991) with hemocytometer results. Finally, reliable quantification of CD4+ cells in healthy human blood via staining with monoclonal antibodies was demonstrated. These results demonstrate the potential of our microfluidic flow cytometry chip as an inexpensive yet high-performance point-of-care device for mobile medicine.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume42
Issue number11
DOIs
StatePublished - Nov 2014

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this