TY - JOUR
T1 - Immunological cross-reactivity to laboratory-produced HPV-11 virions of polysera raised against bacterially derived fusion proteins and synthetic peptides of HPV-6b and HPV-16 capsid proteins
AU - Christensen, Neil D.
AU - Kreider, John W.
AU - Cladel, Nancy M.
AU - Galloway, Denise A.
N1 - Funding Information:
This study was supported by USPHS Grants CA47622 (J.W.K.), CA42791 (D.A.G.), and CA35568 (D.A.G.), and the Jake Gittlen Memorial Golf Tournament. N.D.C. was supported by the Burroughs Welcome fellowship of the American Social Health Association. We thank Pat Welsh, Susan Patrick, and Janette Valentine for excellent technical assistance, and Sandra Eyer for typing the manuscript. We appreciate the assistance of the Wildlife Conservation Officers of the South-East Division of the Pennsylvania Game Commission for providing ear skin from Pennsylvania cottontails for CRPV production.
PY - 1990/3
Y1 - 1990/3
N2 - Polysera raised in rabbits to bacterially derived fusion proteins and synthetic peptides of the Li and L2 ORFs of HPV-6b and -16 were tested for cross-reactivity to laboratory-produced infectious HPV-11 virions. The polysera were analyzed in a series of five different immunological assays including immunoperoxidase staining of the koilocytotic nuclei in sections of formalin-fixed, paraffin-embedded as well as fresh frozen sections of HPV-11 experimental condylomas generated in the athymic nude mouse xenograft system, ELISA, Western blots, and neutralization of infectious HPV-11 virions. ELISA and Western blot assays were used to determine whether the polysera identified external or internal epitopes on HPV-11 virions, and whether there was cross-reactivity to BPV-1 or laboratory-produced CRPV virions. Seven of a total of 12 sera were positive for reactivity to HPV-11 in one or more assays, but none of the reactivity was directed to external epitopes on the intact virions as determined by ELISA. None of the L1 products generated group-specific antigen (GSA) antisera including a synthetic peptide spanning the GSA site. The combination of assays clearly demonstrated that apparent false positive and false negative reactivities of different antisera were obtained for each assay system tested. Thus, no single assay could be used reliably to determine the true antiviral reactivity of a given polysera.
AB - Polysera raised in rabbits to bacterially derived fusion proteins and synthetic peptides of the Li and L2 ORFs of HPV-6b and -16 were tested for cross-reactivity to laboratory-produced infectious HPV-11 virions. The polysera were analyzed in a series of five different immunological assays including immunoperoxidase staining of the koilocytotic nuclei in sections of formalin-fixed, paraffin-embedded as well as fresh frozen sections of HPV-11 experimental condylomas generated in the athymic nude mouse xenograft system, ELISA, Western blots, and neutralization of infectious HPV-11 virions. ELISA and Western blot assays were used to determine whether the polysera identified external or internal epitopes on HPV-11 virions, and whether there was cross-reactivity to BPV-1 or laboratory-produced CRPV virions. Seven of a total of 12 sera were positive for reactivity to HPV-11 in one or more assays, but none of the reactivity was directed to external epitopes on the intact virions as determined by ELISA. None of the L1 products generated group-specific antigen (GSA) antisera including a synthetic peptide spanning the GSA site. The combination of assays clearly demonstrated that apparent false positive and false negative reactivities of different antisera were obtained for each assay system tested. Thus, no single assay could be used reliably to determine the true antiviral reactivity of a given polysera.
UR - https://www.scopus.com/pages/publications/0025349949
UR - https://www.scopus.com/pages/publications/0025349949#tab=citedBy
U2 - 10.1016/0042-6822(90)90180-Y
DO - 10.1016/0042-6822(90)90180-Y
M3 - Article
C2 - 2155503
AN - SCOPUS:0025349949
SN - 0042-6822
VL - 175
SP - 1
EP - 9
JO - Virology
JF - Virology
IS - 1
ER -