TY - JOUR
T1 - Immunoreactivity assay for α-particle emitting monoclonal antibody constructs
AU - Bonavia, Anthony S.
AU - McDevitt, Michael R.
AU - Curcio, Michael J.
AU - Scheinberg, David A.
N1 - Funding Information:
Financial support was derived from NIH R01CA55349, NIHPO1CA33049, Mr. William H. Goodwin and Mrs. Alice Goodwin and the Commonwealth Cancer Foundation for Research, The Experimental Therapeutics Center of Memorial Sloan-Kettering Cencer Center, Doris Duke Charitable Foundation.
PY - 2006/4
Y1 - 2006/4
N2 - Clinical trials using α-particle radiolabeled antibodies require a rapid and reproducible assay of the immunoreactivity of drugs. While live cell assays are typically used to determine the immunoreactive drug fraction, a fixed cell assay may replace the traditional live cell assay and offer the advantages of rapidity, easy availability and consistency for qualifying drugs for preclinical or clinical studies. We have identified optimal cell fixation and immunoreactivity assay conditions and have validated them by performing the fixed-cell assay in clinical trials.
AB - Clinical trials using α-particle radiolabeled antibodies require a rapid and reproducible assay of the immunoreactivity of drugs. While live cell assays are typically used to determine the immunoreactive drug fraction, a fixed cell assay may replace the traditional live cell assay and offer the advantages of rapidity, easy availability and consistency for qualifying drugs for preclinical or clinical studies. We have identified optimal cell fixation and immunoreactivity assay conditions and have validated them by performing the fixed-cell assay in clinical trials.
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U2 - 10.1016/j.apradiso.2005.11.004
DO - 10.1016/j.apradiso.2005.11.004
M3 - Article
C2 - 16352433
AN - SCOPUS:32044447222
SN - 0969-8043
VL - 64
SP - 470
EP - 474
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 4
ER -