TY - JOUR
T1 - Impact of hemicelluloses and pectin on sphere-like bacterial cellulose assembly
AU - Gu, Jin
AU - Catchmark, Jeffrey M.
N1 - Funding Information:
This material is based upon work supported as part of the The Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the U.S. Department of Energy , Office of Science, Office of Basic Energy Sciences under Award Number DE-SC0001. This publication was also supported by the Pennsylvania State University Materials Research Institute Nano Fabrication Network and the National Science Foundation Cooperative Agreement No. 0335765 , National Nanotechnology Infrastructure Network, with Cornell University .
PY - 2012/4/2
Y1 - 2012/4/2
N2 - It has been shown previously that certain strains of the bacterium Gluconacetobacter xylinus produce a spherical form of cellulose where the cellulose was formed in a layered fashion. The spherical cellulose was used as a model system to study cellulose-hemicellulose and cellulose-pectin composite formation. Cultures were produced in the presence of 0.5% (w/v) xyloglucan, xylan, arabinogalactan and pectin under agitating conditions. Cellulose samples with xyloglucan and pectin had different macro structures compared to other culture conditions. The micro structures showed that these two samples formed dense cellulose layers and had fewer cellulose fiber connections between layers. Cellulose samples with xylan and xyloglucan were found to contain more Iβ cellulose as found in higher plants, and exhibited decreases in crystallinity and crystalline sizes according to X-ray diffraction patterns. IR spectroscopy confirmed the changes in crystal allomorph. Cellulose was also grown in cultures containing different blends of both xyloglucan and pectin. Results show that xyloglucan had the dominant impact on the assembly of cellulose, suggesting that xyloglucan and pectin may interact with cellulose at different points in the assembly process, or in different regions. Bacterial cellulose and biomass yields indicated that xyloglucan and pectin could also stimulate the growth of cellulose.
AB - It has been shown previously that certain strains of the bacterium Gluconacetobacter xylinus produce a spherical form of cellulose where the cellulose was formed in a layered fashion. The spherical cellulose was used as a model system to study cellulose-hemicellulose and cellulose-pectin composite formation. Cultures were produced in the presence of 0.5% (w/v) xyloglucan, xylan, arabinogalactan and pectin under agitating conditions. Cellulose samples with xyloglucan and pectin had different macro structures compared to other culture conditions. The micro structures showed that these two samples formed dense cellulose layers and had fewer cellulose fiber connections between layers. Cellulose samples with xylan and xyloglucan were found to contain more Iβ cellulose as found in higher plants, and exhibited decreases in crystallinity and crystalline sizes according to X-ray diffraction patterns. IR spectroscopy confirmed the changes in crystal allomorph. Cellulose was also grown in cultures containing different blends of both xyloglucan and pectin. Results show that xyloglucan had the dominant impact on the assembly of cellulose, suggesting that xyloglucan and pectin may interact with cellulose at different points in the assembly process, or in different regions. Bacterial cellulose and biomass yields indicated that xyloglucan and pectin could also stimulate the growth of cellulose.
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U2 - 10.1016/j.carbpol.2011.12.040
DO - 10.1016/j.carbpol.2011.12.040
M3 - Article
AN - SCOPUS:84857444505
SN - 0144-8617
VL - 88
SP - 547
EP - 557
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 2
ER -