TY - JOUR
T1 - Impact of viral enhancin genes on potency of Lymantria dispar multiple nucleopolyhedrovirus in L. dispar following disruption of the peritrophic matrix
AU - Hoover, Kelli
AU - Humphries, Merideth A.
AU - Gendron, Alyssa R.
AU - Slavicek, James M.
N1 - Funding Information:
We thank Matthew Gardner, Katie Mulfinger, James McNeil, Kim Gilmore, and Karen Bingham for assistance with the bioassays. Funding for this project was provided by a US Forest Service Research Joint Venture Agreement to KH .
PY - 2010/6
Y1 - 2010/6
N2 - Enhancins are metalloproteases found in many betabaculoviruses and several alphabaculoviruses, which enhance alphabaculovirus potency by degrading a protein component of the peritrophic matrix (PM), facilitating passage of virions through this structure. Earlier studies on betabaculovirus enhancins within heterologous systems suggested that enhancins facilitate virion binding to midgut cells. We compared the potency of wild-type Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) with that of single and double enhancin deletion viruses in L. dispar in the presence and absence of an intact PM. Compared to wild-type virus, the double enhancin deletion virus was 6-fold and 14-fold less potent, respectively, indicating that within this homologous system the LdMNPV enhancin genes have a function beyond PM degradation.
AB - Enhancins are metalloproteases found in many betabaculoviruses and several alphabaculoviruses, which enhance alphabaculovirus potency by degrading a protein component of the peritrophic matrix (PM), facilitating passage of virions through this structure. Earlier studies on betabaculovirus enhancins within heterologous systems suggested that enhancins facilitate virion binding to midgut cells. We compared the potency of wild-type Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) with that of single and double enhancin deletion viruses in L. dispar in the presence and absence of an intact PM. Compared to wild-type virus, the double enhancin deletion virus was 6-fold and 14-fold less potent, respectively, indicating that within this homologous system the LdMNPV enhancin genes have a function beyond PM degradation.
UR - http://www.scopus.com/inward/record.url?scp=77954846013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954846013&partnerID=8YFLogxK
U2 - 10.1016/j.jip.2010.02.008
DO - 10.1016/j.jip.2010.02.008
M3 - Article
C2 - 20219469
AN - SCOPUS:77954846013
SN - 0022-2011
VL - 104
SP - 150
EP - 152
JO - Journal of invertebrate pathology
JF - Journal of invertebrate pathology
IS - 2
ER -