Improved method for purification of toxic peptides produced by cyanobacteria

Ken ichi Harada; Makoto Suzuki; Andrew M. Dahlem; Val R. Beasley; Wayne W. Carmichael; Kenneth L. Rinehart

doi:10.1016/0041-0101(88)90182-1

Improved method for purification of toxic peptides produced by cyanobacteria

Ken ichi Harada
, Makoto Suzuki
, Andrew M. Dahlem
, Val R. Beasley
, Wayne W. Carmichael
, Kenneth L. Rinehart

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

K. Harada, M. Suzuki, A. M. Dahlem, V. R. Beasley, W. W. Carmichael and K. L. Rinehart, Jr. Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon26, 433 - 439, 1988. - The improved method consists of ODS-silica gel extraction, and separation on silica gel and HPLC with UV (238 nm) detector. The method has been successfully applied to the isolation of toxic peptides from the Monroe and M-228 strains of Microcystis aeruginosa. This method reduces toxin extraction and separation time, and so enables a rapid isolation of peptide toxins from cyanobacteria.

Original language	English (US)
Pages (from-to)	433-439
Number of pages	7
Journal	Toxicon
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/0041-0101(88)90182-1
State	Published - 1988

All Science Journal Classification (ASJC) codes

Toxicology

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10.1016/0041-0101(88)90182-1

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title = "Improved method for purification of toxic peptides produced by cyanobacteria",

abstract = "K. Harada, M. Suzuki, A. M. Dahlem, V. R. Beasley, W. W. Carmichael and K. L. Rinehart, Jr. Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon26, 433 - 439, 1988. - The improved method consists of ODS-silica gel extraction, and separation on silica gel and HPLC with UV (238 nm) detector. The method has been successfully applied to the isolation of toxic peptides from the Monroe and M-228 strains of Microcystis aeruginosa. This method reduces toxin extraction and separation time, and so enables a rapid isolation of peptide toxins from cyanobacteria.",

author = "Harada, \{Ken ichi\} and Makoto Suzuki and Dahlem, \{Andrew M.\} and Beasley, \{Val R.\} and Carmichael, \{Wayne W.\} and Rinehart, \{Kenneth L.\}",

year = "1988",

doi = "10.1016/0041-0101(88)90182-1",

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T1 - Improved method for purification of toxic peptides produced by cyanobacteria

AU - Harada, Ken ichi

AU - Suzuki, Makoto

AU - Dahlem, Andrew M.

AU - Beasley, Val R.

AU - Carmichael, Wayne W.

AU - Rinehart, Kenneth L.

PY - 1988

Y1 - 1988

N2 - K. Harada, M. Suzuki, A. M. Dahlem, V. R. Beasley, W. W. Carmichael and K. L. Rinehart, Jr. Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon26, 433 - 439, 1988. - The improved method consists of ODS-silica gel extraction, and separation on silica gel and HPLC with UV (238 nm) detector. The method has been successfully applied to the isolation of toxic peptides from the Monroe and M-228 strains of Microcystis aeruginosa. This method reduces toxin extraction and separation time, and so enables a rapid isolation of peptide toxins from cyanobacteria.

AB - K. Harada, M. Suzuki, A. M. Dahlem, V. R. Beasley, W. W. Carmichael and K. L. Rinehart, Jr. Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon26, 433 - 439, 1988. - The improved method consists of ODS-silica gel extraction, and separation on silica gel and HPLC with UV (238 nm) detector. The method has been successfully applied to the isolation of toxic peptides from the Monroe and M-228 strains of Microcystis aeruginosa. This method reduces toxin extraction and separation time, and so enables a rapid isolation of peptide toxins from cyanobacteria.

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SP - 433

EP - 439

JO - Toxicon

JF - Toxicon

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ER -

Improved method for purification of toxic peptides produced by cyanobacteria

Abstract

All Science Journal Classification (ASJC) codes

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