TY - JOUR
T1 - Improved method of in vitro regeneration in Leucaena leucocephala - a leguminous pulpwood tree species
AU - Shaik, Noor M.
AU - Arha, Manish
AU - Nookaraju, A.
AU - Gupta, Sushim K.
AU - Srivastava, Sameer
AU - Yadav, Arun K.
AU - Kulkarni, Pallavi S.
AU - Abhilash, O. U.
AU - Vishwakarma, Rishi K.
AU - Singh, Somesh
AU - Tatkare, Rajeshri
AU - Chinnathambi, Kannan
AU - Rawal, Shuban K.
AU - Khan, Bashir M.
N1 - Funding Information:
Financial support in the form of Junior Research Fellowship to N.M. Shaik, M. Arha, S.K. Gupta, P.S. Kulkarni, O.U. Abhilash, T. Rajeshri, Kannan Chinathambi and A. Nookaraju by the Council of Scientific and Industrial Research (CSIR), New Delhi; by University Grants Commission (UGC), New Delhi, India to S. Srivastava and A.K. Yadav is gratefully acknowledged.
PY - 2009/10
Y1 - 2009/10
N2 - Leucaena leucocephala is a fast growing multipurpose legume tree used for forage, leaf manure, paper and pulp. Lignin in Leucaena pulp adversely influences the quality of paper produced. Developing transgenic Leucaena with altered lignin by genetic engineering demands an optimized regeneration system. The present study deals with optimization of regeneration system for L. leucocephala cv. K636. Multiple shoot induction from the cotyledonary nodes of L. leucocephala was studied in response to cytokinins, thidiazuron (TDZ) and N6-benzyladenine (BA) supplemented in half strength MS (1/2-MS) medium and also their effect on in vitro rooting of the regenerated shoots. Multiple shoots were induced from cotyledonary nodes at varied frequencies depending on the type and concentration of cytokinin used in the medium. TDZ was found to induce more number of shoots per explant than BA, with a maximum of 7 shoots at an optimum concentration of 0.23 μM. Further increase in TDZ concentration resulted in reduced shoot length and fasciation of the shoots. Liquid pulse treatment of the explants with TDZ did not improve the shoot production further but improved the subsequent rooting of the shoots that regenerated. Regenerated shoots successfully rooted on 1/2-MS medium supplemented with 0.54 μM α-naphthaleneacetic acid (NAA). Rooted shoots of Leucaena were transferred to coco-peat and hardened plantlets showed ≥ 90 % establishment in the green house.
AB - Leucaena leucocephala is a fast growing multipurpose legume tree used for forage, leaf manure, paper and pulp. Lignin in Leucaena pulp adversely influences the quality of paper produced. Developing transgenic Leucaena with altered lignin by genetic engineering demands an optimized regeneration system. The present study deals with optimization of regeneration system for L. leucocephala cv. K636. Multiple shoot induction from the cotyledonary nodes of L. leucocephala was studied in response to cytokinins, thidiazuron (TDZ) and N6-benzyladenine (BA) supplemented in half strength MS (1/2-MS) medium and also their effect on in vitro rooting of the regenerated shoots. Multiple shoots were induced from cotyledonary nodes at varied frequencies depending on the type and concentration of cytokinin used in the medium. TDZ was found to induce more number of shoots per explant than BA, with a maximum of 7 shoots at an optimum concentration of 0.23 μM. Further increase in TDZ concentration resulted in reduced shoot length and fasciation of the shoots. Liquid pulse treatment of the explants with TDZ did not improve the shoot production further but improved the subsequent rooting of the shoots that regenerated. Regenerated shoots successfully rooted on 1/2-MS medium supplemented with 0.54 μM α-naphthaleneacetic acid (NAA). Rooted shoots of Leucaena were transferred to coco-peat and hardened plantlets showed ≥ 90 % establishment in the green house.
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U2 - 10.1007/s12298-009-0035-5
DO - 10.1007/s12298-009-0035-5
M3 - Article
C2 - 23572941
AN - SCOPUS:77954045113
SN - 0971-5894
VL - 15
SP - 311
EP - 318
JO - Physiology and Molecular Biology of Plants
JF - Physiology and Molecular Biology of Plants
IS - 4
ER -