Abstract
This paper describes an analytical method for the assessment of tobacco-specific N-nitrosamines (TSNA) in tobacco which involves supercritical fluid extraction and quantification by gas chromatography with thermal energy analyzer detection. Extraction of tobacco with supercritical carbon dioxide, to which 10% methanol is added, is followed by a cleanup on silica cartridges which separates the TSNA from the bulk of other tobacco components present in the extract. Gas chromatographic separation is performed on a DB-5 fused silica capillary column, and quantitative results are ascertained by using 4-(ethylnitrosamino)-1-(3-pyridyl)-1-butanone as an internal standard. This method is quantitative and well reproducible and meets the qualifications to be chosen as a standardized analytical method.
Original language | English (US) |
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Pages (from-to) | 916-922 |
Number of pages | 7 |
Journal | Journal of agricultural and food chemistry |
Volume | 43 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1 1995 |
All Science Journal Classification (ASJC) codes
- General Chemistry
- General Agricultural and Biological Sciences