Improved microchip design and application for in situ transmission electron microscopy of macromolecules

Madeline J. Dukes, Rebecca Thomas, John Damiano, Kate L. Klein, Sharavanan Balasubramaniam, Sanem Kayandan, Judy S. Riffle, Richey M. Davis, Sarah M. McDonald, Deborah F. Kelly

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Understanding the fundamental properties of macromolecules has enhanced the development of emerging technologies used to improve biomedical research. Currently, there is a critical need for innovative platforms that can illuminate the function of biomedical reagents in a native environment. To address this need, we have developed an in situ approach to visualize the dynamic behavior of biomedically relevant macromolecules at the nanoscale. Newly designed silicon nitride devices containing integrated microwells were used to enclose active macromolecular specimens in liquid for transmission electron microscopy imaging purposes.We were able to successfully examine novel magnetic resonance imaging contrast reagents, micelle suspensions, liposome carrier vehicles, and transcribing viral assemblies. With each specimen tested, the integrated microwells adequately maintained macromolecules in discrete local environments while enabling thin liquid layers to be produced.

Original languageEnglish (US)
Pages (from-to)338-345
Number of pages8
JournalMicroscopy and Microanalysis
Issue number2
StatePublished - Apr 2014

All Science Journal Classification (ASJC) codes

  • Instrumentation


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