Improved radioimmunoassay for 11-deoxycortisol (compound S) in plasma

L. M. Demers, L. Ebright, D. D. Derck

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Described is a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9 μg/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 μg/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean±2SD) of 0.5 to 12.0 μg/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve.

Original languageEnglish (US)
Pages (from-to)1704-1707
Number of pages4
JournalClinical chemistry
Issue number10
StatePublished - 1979

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical


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