TY - JOUR
T1 - Improved radioimmunoassay for 11-deoxycortisol (compound S) in plasma
AU - Demers, L. M.
AU - Ebright, L.
AU - Derck, D. D.
PY - 1979
Y1 - 1979
N2 - Described is a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9 μg/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 μg/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean±2SD) of 0.5 to 12.0 μg/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve.
AB - Described is a radioimmunoassay for 11-deoxycortisol (Compound S) that involves preliminary chromatography on Sephadex LH-20, to isolate 11-deoxycortisol from crossreacting structurally related steroids. Pools with means of 3.4, 12.9 μg/L gave intra-assay coefficients of variation of 9.7, 9.6, and 11.4%, respectively; pools with means of 4.6, 27, and 95 μg/L gave inter-assay CV's of 10.1, 9.7, and 6.2%, respectively. A normal range (mean±2SD) of 0.5 to 12.0 μg/L was obtained. This procedure is useful for the specific determination of 11-deoxycortisol, commonly determined in blood as part of the metyrapone test for assessing pituitary corticotropin reserve.
UR - http://www.scopus.com/inward/record.url?scp=0018691605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018691605&partnerID=8YFLogxK
U2 - 10.1093/clinchem/25.10.1704
DO - 10.1093/clinchem/25.10.1704
M3 - Article
C2 - 476918
AN - SCOPUS:0018691605
SN - 0009-9147
VL - 25
SP - 1704
EP - 1707
JO - Clinical chemistry
JF - Clinical chemistry
IS - 10
ER -