In silico design and adaptive evolution of Escherichia coli for production of lactic acid

Stephen S. Fong; Anthony P. Burgard; Christopher D. Herring; Eric M. Knight; Frederick R. Blattner; Costas D. Maranas; Bernhard O. Palsson

doi:10.1002/bit.20542

In silico design and adaptive evolution of Escherichia coli for production of lactic acid

Stephen S. Fong, Anthony P. Burgard, Christopher D. Herring, Eric M. Knight, Frederick R. Blattner, Costas D. Maranas, Bernhard O. Palsson

Research output: Contribution to journal › Article › peer-review

319 Scopus citations

Abstract

The development and validation of new methods to help direct rational strain design for metabolite overproduction remains an important problem in metabolic engineering. Here we show that computationally predicted E. coli strain designs, calculated from a genome-scale metabolic model, can lead to successful production strains and that adaptive evolution of the engineered strains can lead to improved production capabilities. Three strain designs for lactate production were implemented yielding a total of 11 evolved production strains that were used to demonstrate the utility of this integrated approach. Strains grown on 2 g/L glucose at 37°C showed lactate titers ranging from 0.87 to 1.75 g/L and secretion rates that were directly coupled to growth rates.

Original language	English (US)
Pages (from-to)	643-648
Number of pages	6
Journal	Biotechnology and bioengineering
Volume	91
Issue number	5
DOIs	https://doi.org/10.1002/bit.20542
State	Published - Sep 5 2005

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "The development and validation of new methods to help direct rational strain design for metabolite overproduction remains an important problem in metabolic engineering. Here we show that computationally predicted E. coli strain designs, calculated from a genome-scale metabolic model, can lead to successful production strains and that adaptive evolution of the engineered strains can lead to improved production capabilities. Three strain designs for lactate production were implemented yielding a total of 11 evolved production strains that were used to demonstrate the utility of this integrated approach. Strains grown on 2 g/L glucose at 37°C showed lactate titers ranging from 0.87 to 1.75 g/L and secretion rates that were directly coupled to growth rates.",

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AU - Blattner, Frederick R.

AU - Maranas, Costas D.

AU - Palsson, Bernhard O.

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In silico design and adaptive evolution of Escherichia coli for production of lactic acid

Abstract

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