In vitro cellular effects of hematoporphyrin derivative

Michael W. Berns, Robert Burns, Daryn Sperling, Mark Guiltinan, Ann Siemens, Robert Walter, William Wright, Marie Hammer-Wilson, Alan Wile, Anton Dahlman, Fred, M. Johnson

Research output: Contribution to journalArticlepeer-review

203 Scopus citations


Several in vitro cell systems were exposed to hematoporphyrin derivative (HPD): established lines of rat kangaroo epithelial kidney; normal mouse embryonic fibroblasts; and differentiated neonatal rat myocardial cells. The uptake of HPD (25 to 100 µg/ml) by individual cells occurred rapidly over a 2-hr period and leveled off by 24 hr. HPD was excreted from cells by 48 hr after exposure. However, a low level of HPD (above background) was maintained in cells for up to 4 days following cessation of exposure. Intracellular binding of HPD was to mitochondria as demonstrated by fluorescence microscopy. HPD was also shown to have a growth-inhibiting effect on rat kangaroo cells without added light. The growth effects on mouse cells were less marked.

Original languageEnglish (US)
Pages (from-to)2325-2329
Number of pages5
JournalCancer Research
Issue number6
StatePublished - Jun 1 1982

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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