In vitro cellular effects of hematoporphyrin derivative

Michael W. Berns; Robert Burns; Daryn Sperling; Mark Guiltinan; Ann Siemens; Robert Walter; William Wright; Marie Hammer-Wilson; Alan Wile; Anton Dahlman; Fred, M. Johnson

In vitro cellular effects of hematoporphyrin derivative

Michael W. Berns, Robert Burns, Daryn Sperling, Mark Guiltinan, Ann Siemens, Robert Walter, William Wright, Marie Hammer-Wilson, Alan Wile, Anton Dahlman, Fred, M. Johnson

Plant Science

Research output: Contribution to journal › Article › peer-review

203 Scopus citations

Abstract

Several in vitro cell systems were exposed to hematoporphyrin derivative (HPD): established lines of rat kangaroo epithelial kidney; normal mouse embryonic fibroblasts; and differentiated neonatal rat myocardial cells. The uptake of HPD (25 to 100 µg/ml) by individual cells occurred rapidly over a 2-hr period and leveled off by 24 hr. HPD was excreted from cells by 48 hr after exposure. However, a low level of HPD (above background) was maintained in cells for up to 4 days following cessation of exposure. Intracellular binding of HPD was to mitochondria as demonstrated by fluorescence microscopy. HPD was also shown to have a growth-inhibiting effect on rat kangaroo cells without added light. The growth effects on mouse cells were less marked.

Original language	English (US)
Pages (from-to)	2325-2329
Number of pages	5
Journal	Cancer Research
Volume	42
Issue number	6
State	Published - Jun 1 1982

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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Cite this

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AU - Berns, Michael W.

AU - Burns, Robert

AU - Sperling, Daryn

AU - Guiltinan, Mark

AU - Siemens, Ann

AU - Walter, Robert

AU - Wright, William

AU - Hammer-Wilson, Marie

AU - Wile, Alan

AU - Dahlman, Anton

AU - Johnson, Fred, M.

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N2 - Several in vitro cell systems were exposed to hematoporphyrin derivative (HPD): established lines of rat kangaroo epithelial kidney; normal mouse embryonic fibroblasts; and differentiated neonatal rat myocardial cells. The uptake of HPD (25 to 100 µg/ml) by individual cells occurred rapidly over a 2-hr period and leveled off by 24 hr. HPD was excreted from cells by 48 hr after exposure. However, a low level of HPD (above background) was maintained in cells for up to 4 days following cessation of exposure. Intracellular binding of HPD was to mitochondria as demonstrated by fluorescence microscopy. HPD was also shown to have a growth-inhibiting effect on rat kangaroo cells without added light. The growth effects on mouse cells were less marked.

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In vitro cellular effects of hematoporphyrin derivative

Abstract

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