TY - JOUR
T1 - In vitro characterization and micromechanics of tumor cell chemotactic protrusion, locomotion, and extravasation
AU - Dong, Cheng
AU - Slattery, Margaret J.
AU - Rank, Bradley M.
AU - You, Jun
N1 - Funding Information:
The authors acknowledge Dr. Scott Simon at University of California Davis for providing transfected mouse L cells; Dr. Michael Lawrence at University of Virginia for providing Jurkat cells; Dr. Lance Liotta at NCI/NIH and Dr. Danny Welch at Penn State Medical Center for providing human melanoma cell lines. Experimental assistance from Karen Trippett and Louis Hodgson is greatly appreciated. This work was supported by grants from NSF-BES-9502069 and NIH-CA-76434, and in part by the Innovative Biotechnology Research Fund from the Pennsylvania State University-Life Science Consortium.
PY - 2002
Y1 - 2002
N2 - The objective of this paper is to introduce some novel in vitro applications in characterizing human melanoma cell protrusion and migration in response to soluble extracellular matrix protein stimulation. Specifically, we describe two assay systems: (1) dual-micropipette manipulation and (2) flow-migration chamber. Applications of the dual-micropipet technique provided kinetic measure of cell movement, cyclic pseudopod protrusion, and subsequent cell locomotion governed by chemotactic molecular transport dynamics. Chemotactic concentration gradient was found to influence significantly pseudopod protrusion frequency and locomotion speed, but not the protrusion extension. To further characterize active tumor cell extravasation, a process that involves dynamic tumor cell adhesion to vascular endothelium under flow conditions and subsequent transendothelial migration in response to chemotactic signals from the interstitial space, we developed a flow-migration chemotaxis system. This assay enabled characterization of tumor cell transcellular migration in terms of chemotactic signal gradients, shear forces, and cell-substrate adhesion. Results suggest that shear flow plays significant roles in tumor cell extravasation that is regulated by both tumor cell motility and tumor cell adhesion to endothelial molecules in a cooperative Process.
AB - The objective of this paper is to introduce some novel in vitro applications in characterizing human melanoma cell protrusion and migration in response to soluble extracellular matrix protein stimulation. Specifically, we describe two assay systems: (1) dual-micropipette manipulation and (2) flow-migration chamber. Applications of the dual-micropipet technique provided kinetic measure of cell movement, cyclic pseudopod protrusion, and subsequent cell locomotion governed by chemotactic molecular transport dynamics. Chemotactic concentration gradient was found to influence significantly pseudopod protrusion frequency and locomotion speed, but not the protrusion extension. To further characterize active tumor cell extravasation, a process that involves dynamic tumor cell adhesion to vascular endothelium under flow conditions and subsequent transendothelial migration in response to chemotactic signals from the interstitial space, we developed a flow-migration chemotaxis system. This assay enabled characterization of tumor cell transcellular migration in terms of chemotactic signal gradients, shear forces, and cell-substrate adhesion. Results suggest that shear flow plays significant roles in tumor cell extravasation that is regulated by both tumor cell motility and tumor cell adhesion to endothelial molecules in a cooperative Process.
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U2 - 10.1114/1.1468889
DO - 10.1114/1.1468889
M3 - Article
C2 - 12051619
AN - SCOPUS:0035986936
SN - 0090-6964
VL - 30
SP - 344
EP - 355
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 3
ER -