TY - JOUR
T1 - In vitro characterization of polyesters of aconitic acid, glycerol, and cinnamic acid for bone tissue engineering
AU - Kanitkar, Akanksha
AU - Chen, Cong
AU - Smoak, Mollie
AU - Hogan, Katie
AU - Scherr, Thomas
AU - Aita, Giovanna
AU - Hayes, Daniel
N1 - Publisher Copyright:
© The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
PY - 2015/3/26
Y1 - 2015/3/26
N2 - In this study, a novel class of polyesters of glycerol, aconitic acid, and cinnamic acid were synthesized along with their hydroxyapatite (HA) composites, and studied for their potential application in bone defect repair. An osteogenic study was conducted with human adipose derived mesenchymal stem cells (hASCs) to determine the osteoinductive ability of aconitic acid-glycerol (AG) polyesters, AG:HA (80:20), aconitic acid-glycerol-cinnamic acid (AGC) polyesters, and AGC:HA (80:20) to serve as bone scaffolds. The results indicate that AGC scaffolds have the highest mechanical strength in comparison to AG, AG:HA (80:20), and AGC:HA (80:20) scaffolds due to its low porosity. It was determined by cytotoxicity and osteogenesis experiments that hASCs cultured for 21 days on AG:HA (80:20) scaffolds in stromal medium exhibited a greater number of live cells than control PCL:HA composites. Moreover, hASCs cultured on foamed AG:HA (80:20) scaffolds resulted in the highest levels of mineralization, increased alkaline phosphatase (ALP) expression, and the greatest osteocalcin (OCN) expression after 21 days. Overall, AG:HA (100:0 and 80:20) scaffolds had higher mechanical strength and cytocompatibility than the PCL:HA control. In vitro osteogenic study demonstrated that AG:HA (100:0 and 80:20) synthesized using sugarcane industry by-products hold potential as scaffolds for bone tissue engineering applications.
AB - In this study, a novel class of polyesters of glycerol, aconitic acid, and cinnamic acid were synthesized along with their hydroxyapatite (HA) composites, and studied for their potential application in bone defect repair. An osteogenic study was conducted with human adipose derived mesenchymal stem cells (hASCs) to determine the osteoinductive ability of aconitic acid-glycerol (AG) polyesters, AG:HA (80:20), aconitic acid-glycerol-cinnamic acid (AGC) polyesters, and AGC:HA (80:20) to serve as bone scaffolds. The results indicate that AGC scaffolds have the highest mechanical strength in comparison to AG, AG:HA (80:20), and AGC:HA (80:20) scaffolds due to its low porosity. It was determined by cytotoxicity and osteogenesis experiments that hASCs cultured for 21 days on AG:HA (80:20) scaffolds in stromal medium exhibited a greater number of live cells than control PCL:HA composites. Moreover, hASCs cultured on foamed AG:HA (80:20) scaffolds resulted in the highest levels of mineralization, increased alkaline phosphatase (ALP) expression, and the greatest osteocalcin (OCN) expression after 21 days. Overall, AG:HA (100:0 and 80:20) scaffolds had higher mechanical strength and cytocompatibility than the PCL:HA control. In vitro osteogenic study demonstrated that AG:HA (100:0 and 80:20) synthesized using sugarcane industry by-products hold potential as scaffolds for bone tissue engineering applications.
UR - http://www.scopus.com/inward/record.url?scp=84923464381&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923464381&partnerID=8YFLogxK
U2 - 10.1177/0885328214553961
DO - 10.1177/0885328214553961
M3 - Article
C2 - 25281649
AN - SCOPUS:84923464381
SN - 0885-3282
VL - 29
SP - 1075
EP - 1085
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 8
ER -