In Vitro Cultivation of the Hymenoptera Genetic Model, Nasonia

Robert M. Brucker, Seth R. Bordenstein

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The wasp genus Nasonia is a genetic model with unique advantages for the study of interspecific differences, including haplodiploidy and interfertile species. However, as a parasitoid, Nasonia is confined within a fly host, thus restricting direct observations and manipulation of development over time. Here, we present the first in vitro cultivation method for this system that decouples Nasonia from its host, allowing continuous observations from embryo to adulthood. Using transwell plates and a simple Nasonia rearing medium, we demonstrate a technique that will significantly expand the utility of the Nasonia model.

Original languageEnglish (US)
Article numbere51269
JournalPloS one
Issue number12
StatePublished - Dec 5 2012

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General


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