In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity

Nushrat J. Hoque, Madison P. Helm, Emily E. Weinert

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems. Here, we describe a method for anaerobic quantification of cyclic di-GMP production which can be used to measure the impact of molecular oxygen, nitric oxide, and carbon monoxide on the catalysis of a diguanylate cyclase-containing protein and the possible pitfalls in the experimental procedure.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-86
Number of pages12
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2648
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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