@inbook{03c9cf632b94422da17c8f7ac9fab7ae,
title = "In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity",
abstract = "Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems. Here, we describe a method for anaerobic quantification of cyclic di-GMP production which can be used to measure the impact of molecular oxygen, nitric oxide, and carbon monoxide on the catalysis of a diguanylate cyclase-containing protein and the possible pitfalls in the experimental procedure.",
author = "Hoque, {Nushrat J.} and Helm, {Madison P.} and Weinert, {Emily E.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3080-8_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "75--86",
booktitle = "Methods in Molecular Biology",
}