In vitro palmitoylation of native bovine brain Goα

Shengyu Yang, Youguo Huang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The native Goα was purified from bovine brain cortex and palmitoylated in vitro. The in vitro palmitoylation site was the same as that in vivo. The internal palmitoylation of purified native Goα was found to be largely maintained. The apparant palmitoylation ratio was significantly increased after the Goα was treated with DTT. The GTPγS binding characteristic of Goα was not influenced by palmitoylation, however, the affinity for LUVs was increased dramatically. The in vitro palmitoylation model of Goα provides a better basis for studying the functional role of G protein palmitoylation in signal transduction.

Original languageEnglish (US)
Pages (from-to)486-488
Number of pages3
JournalScience in China, Series C: Life Sciences
Issue number5
StatePublished - Oct 2000

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Environmental Science
  • General Agricultural and Biological Sciences


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