In-vivo acoustic imaging of thermally induced changes of a single cell

C. Miyasaka; Bernhard R. Tittmann

In-vivo acoustic imaging of thermally induced changes of a single cell

C. Miyasaka, Bernhard R. Tittmann

Research output: Contribution to journal › Conference article › peer-review

Abstract

In-vivo acoustic microscopy with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the Scanning Acoustic Microscope (SAM). The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g., shrinkage, volume change or lift-off) were clearly visualized and measured.

Original language	English (US)
Pages (from-to)	1645-1648
Number of pages	4
Journal	Proceedings of the IEEE Ultrasonics Symposium
Volume	2
State	Published - Dec 1 2002
Event	2002 IEEE Ultrasonics Symposium - Munich, Germany Duration: Oct 8 2002 → Oct 11 2002

All Science Journal Classification (ASJC) codes

Acoustics and Ultrasonics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "In-vivo acoustic imaging of thermally induced changes of a single cell",

abstract = "In-vivo acoustic microscopy with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the Scanning Acoustic Microscope (SAM). The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g., shrinkage, volume change or lift-off) were clearly visualized and measured.",

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AU - Miyasaka, C.

AU - Tittmann, Bernhard R.

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N2 - In-vivo acoustic microscopy with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the Scanning Acoustic Microscope (SAM). The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g., shrinkage, volume change or lift-off) were clearly visualized and measured.

AB - In-vivo acoustic microscopy with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the Scanning Acoustic Microscope (SAM). The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g., shrinkage, volume change or lift-off) were clearly visualized and measured.

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JF - Proceedings of the IEEE Ultrasonics Symposium

T2 - 2002 IEEE Ultrasonics Symposium

Y2 - 8 October 2002 through 11 October 2002

ER -

In-vivo acoustic imaging of thermally induced changes of a single cell

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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