TY - JOUR
T1 - In vivo depletion and genetic targeting of mouse intestinal CX3CR1+ mononuclear phagocytes
AU - Koscsó, Balázs
AU - Gowda, Kavitha
AU - Bogunovic, Milena
N1 - Funding Information:
We would like to thank the members of the Immunological Genome Project ( www.immgen.org ) including Miriam Merad laboratory (Icahn School of Medicine at Mount Sinai) and the members of Flow Cytometry Core Facility in the Department of Comparative Medicine and Department of Pathology at Penn State College of Medicine for their assistance with our experiments. This work was funded, in part, by grants from Crohn's and Colitis Foundation of America (M.B.) ( 3055 ) and the Pennsylvania Department of Health ( 4100068724 ) using Tobacco CURE Funds (M.B.). The Pennsylvania Department of Health specifically disclaims responsibility for any analyses, interpretations or conclusions.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1+ MP subsets. The described tools might be useful for studies of CX3CR1+ MP function in various murine experimental models, particularly under non-inflammatory conditions.
AB - Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1+ MP subsets. The described tools might be useful for studies of CX3CR1+ MP function in various murine experimental models, particularly under non-inflammatory conditions.
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U2 - 10.1016/j.jim.2015.12.009
DO - 10.1016/j.jim.2015.12.009
M3 - Article
C2 - 26705686
AN - SCOPUS:84953233387
SN - 0022-1759
VL - 432
SP - 13
EP - 23
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -