TY - JOUR
T1 - Inactivation of the bacterial RNA polymerase due to acquisition of secondary structure by theω subunit
AU - Sarkar, Paramita
AU - Sardesai, Abhijit A.
AU - Murakami, Katsuhiko S.
AU - Chatterji, Dipankar
PY - 2013/8/30
Y1 - 2013/8/30
N2 - Background: Although universally conserved, ω is the only nonessential subunit of RNA polymerase, and its function is mostly undetermined. Results: Dominant lethal mutants of ω were isolated and analyzed genetically and biochemically. Conclusion: Mutations in ω that alter its structure interfere with RNA polymerase catalytic properties. Significance: Results suggest a critical role for ω during transcription, bypassing the effects of compensatory proteins.
AB - Background: Although universally conserved, ω is the only nonessential subunit of RNA polymerase, and its function is mostly undetermined. Results: Dominant lethal mutants of ω were isolated and analyzed genetically and biochemically. Conclusion: Mutations in ω that alter its structure interfere with RNA polymerase catalytic properties. Significance: Results suggest a critical role for ω during transcription, bypassing the effects of compensatory proteins.
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U2 - 10.1074/jbc.M113.468520
DO - 10.1074/jbc.M113.468520
M3 - Article
C2 - 23843456
AN - SCOPUS:84883411070
SN - 0021-9258
VL - 288
SP - 25076
EP - 25087
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -