Increased 12-HETE production in bovine lymphocytes during selenium deficiency

Jane F. Maddox, Yu Zhang Cao, Andrea M. Mastro, Richard W. Scholz, George Hildenbrandt, C. Channa Reddy

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6 Scopus citations


When peripheral blood lymphocytes were incubated with arachidonic acid in the presence of Ca++ ionophore (A23187), the cells from the selenium-deficient dairy cows produced significantly greater quantities of 12-hydroxyeicosatetraenoic acid (12-HETE) than the cells from the selenium-supplemented animals. The major product of reaction was verified as 12-HETE by cochromatography with a 12-HETE standard on an HPLC and structural analysis by GC-MS. Additionally, concanavalin A-stimulated lymphocyte proliferation was significantly decreased in cells from the Se-deficient cows. Furthermore, 12-HETE generated by the A23187-stimulated lymphocytes inhibited lymphocyte proliferation when added to Se-supplemented cell cultures. These observations suggest that self-regulation of lymphocyte proliferation might be mediated by 12-HETE production, especially during an altered nutritional state such as Se deficiency.

Original languageEnglish (US)
Pages (from-to)389-395
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 27 1991

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology


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