Increased Binding of [3H]GABA to Striatal Membranes Following Ischemia

Andrew Francis; William Pulsinelli

doi:10.1111/j.1471-4159.1983.tb13598.x

Increased Binding of [³H]GABA to Striatal Membranes Following Ischemia

Andrew Francis, William Pulsinelli

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Abstract

Abstract: Sodium‐independent binding of [³H]γ‐aminobutyric acid ([³H]GABA) to membranes prepared from ischemic‐damaged rat striatum was studied by kinetic and time‐course analysis. Three days after 40 min of ischemia, [³H]GABA binding increased fourfold over control values. Scatchard analysis of the binding showed that ischemia significantly increased the affinity (K_D) and the total number of binding sites (B_max) for the high‐affinity GABA receptor. These results support the conclusion that transient forebrain ischemia damages striatal GABAergic neurons. 1983 International Society for Neurochemistry

Original language	English (US)
Pages (from-to)	1497-1499
Number of pages	3
Journal	Journal of neurochemistry
Volume	40
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-4159.1983.tb13598.x
State	Published - May 1983

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1983.tb13598.x

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abstract = "Abstract: Sodium‐independent binding of [3H]γ‐aminobutyric acid ([3H]GABA) to membranes prepared from ischemic‐damaged rat striatum was studied by kinetic and time‐course analysis. Three days after 40 min of ischemia, [3H]GABA binding increased fourfold over control values. Scatchard analysis of the binding showed that ischemia significantly increased the affinity (KD) and the total number of binding sites (Bmax) for the high‐affinity GABA receptor. These results support the conclusion that transient forebrain ischemia damages striatal GABAergic neurons. 1983 International Society for Neurochemistry",

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AU - Pulsinelli, William

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AB - Abstract: Sodium‐independent binding of [3H]γ‐aminobutyric acid ([3H]GABA) to membranes prepared from ischemic‐damaged rat striatum was studied by kinetic and time‐course analysis. Three days after 40 min of ischemia, [3H]GABA binding increased fourfold over control values. Scatchard analysis of the binding showed that ischemia significantly increased the affinity (KD) and the total number of binding sites (Bmax) for the high‐affinity GABA receptor. These results support the conclusion that transient forebrain ischemia damages striatal GABAergic neurons. 1983 International Society for Neurochemistry

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Increased Binding of [³H]GABA to Striatal Membranes Following Ischemia

Abstract

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