@article{471a84d322994721b5f2a4485320c4d7,
title = "Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion",
abstract = "Objectives: To carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant. Results: The first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a dexamethasone activatable chimeric transcription factor. This four-fold enhancement in embryo production rate utilized a glucocorticoid receptor fused to an embryogenic transcription factor LEAFY COTYLEDON 2. Where previous T. cacao somatic embryogenesis has been restricted to dissected flower parts, this construct confers an unprecedented embryogenic potential to leaves. Conclusions: Activatable chimeric transcription factors provide a means for elucidating the regulatory cascade associated with plant somatic embryogenesis towards improving its use for somatic regeneration of transgenics and plant propagation.",
author = "Shires, {Morgan E.} and Florez, {Sergio L.} and Lai, {Tina S.} and Curtis, {Wayne R.}",
note = "Funding Information: Acknowledgements This research was initiated under National Science Foundation{\textquoteright}s Chemical, Bioengineering, Environmental, and Transport Systems CBET Grant #1035072 and completed under National Science Foundation{\textquoteright}s Basic Research to Enhance Agricultural Development (NSF BREAD) program Grant number 1543929. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The American Cocoa Research Foundation Endowment in the Molecular Biology of Cacao at Penn State to Drs. Mark Guiltinan and Siela Maximova also provided support for the execution of this work including cacao transgenic plant infrastructure. Additional acknowledgements and author contributions are provided following Supplementary Table 1. Funding Information: This research was initiated under National Science Foundation?s Chemical, Bioengineering, Environmental, and Transport Systems CBET Grant #1035072 and completed under National Science Foundation?s Basic Research to Enhance Agricultural Development (NSF BREAD) program Grant number 1543929. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The American Cocoa Research Foundation Endowment in the Molecular Biology of Cacao at Penn State to Drs. Mark Guiltinan and Siela Maximova also provided support for the execution of this work including cacao transgenic plant infrastructure. Additional acknowledgements and author contributions are provided following Supplementary Table?1. Supplementary Table?1?qPCR primer sequences. Supplementary Table?2?Cacao (Theobroma cacao) Primary Callus Growth Medium (PCG). Supplementary Table?3?Cacao (Theobroma cacao) Secondary Callus Growth Medium (E5B). Supplementary Table?4?Cacao (Theobroma cacao) Embryo Development (ED) Medium. Supplementary Table?5?Primary Embryo Conversion (PEC) Medium. Supplementary Table?6?Root Development (RD) Medium. Supplementary Figure?1?Transgenic Theobroma cacao plant containing the homologous TcLEC2 gene fusion with the glucocorticoid receptor (TcLEC2-GR) under the control of the constitutive 35S CaMV promoter. Supplementary Figure?2?T. cacao transgenic LEC2-GR secondary embryos generated from the initial transformant (left), as compared to embryos derived from the leaf of the regenerated transgenic LEC2-GR plant (right) illustrating the smaller size at which embryos would tend to develop on the same embryo development media. Morgan Shires and Sergio Florez have contributed equally to this work. Publisher Copyright: {\textcopyright} 2017, The Author(s).",
year = "2017",
month = nov,
day = "1",
doi = "10.1007/s10529-017-2404-4",
language = "English (US)",
volume = "39",
pages = "1747--1755",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "11",
}