Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda; Diane Schloeder; Stephen J. Benkovic; Richard A. Lerner

doi:10.1126/science.3413482

Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda, Diane Schloeder, Stephen J. Benkovic, Richard A. Lerner

Chemistry

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220 Scopus citations

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Original language	English (US)
Pages (from-to)	1188-1191
Number of pages	4
Journal	Science
Volume	241
Issue number	4870
DOIs	https://doi.org/10.1126/science.3413482
State	Published - 1988

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title = "Induction of an antibody that catalyzes the hydrolysis of an amide bond",

abstract = "Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.",

author = "Janda, {Kim D.} and Diane Schloeder and Benkovic, {Stephen J.} and Lerner, {Richard A.}",

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AU - Schloeder, Diane

AU - Benkovic, Stephen J.

AU - Lerner, Richard A.

PY - 1988

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N2 - Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

AB - Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

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ER -

Induction of an antibody that catalyzes the hydrolysis of an amide bond

Abstract

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