Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda; Diane Schloeder; Stephen J. Benkovic; Richard A. Lerner

doi:10.1126/science.3413482

Induction of an antibody that catalyzes the hydrolysis of an amide bond

Kim D. Janda
, Diane Schloeder
, Stephen J. Benkovic
, Richard A. Lerner

Chemistry

Research output: Contribution to journal › Article › peer-review

224 Scopus citations

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Original language	English (US)
Pages (from-to)	1188-1191
Number of pages	4
Journal	Science
Volume	241
Issue number	4870
DOIs	https://doi.org/10.1126/science.3413482
State	Published - 1988

All Science Journal Classification (ASJC) codes

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title = "Induction of an antibody that catalyzes the hydrolysis of an amide bond",

abstract = "Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.",

author = "Janda, \{Kim D.\} and Diane Schloeder and Benkovic, \{Stephen J.\} and Lerner, \{Richard A.\}",

year = "1988",

doi = "10.1126/science.3413482",

language = "English (US)",

volume = "241",

pages = "1188--1191",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4870",

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AU - Janda, Kim D.

AU - Schloeder, Diane

AU - Benkovic, Stephen J.

AU - Lerner, Richard A.

PY - 1988

Y1 - 1988

N2 - Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

AB - Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

UR - https://www.scopus.com/pages/publications/0023759322

UR - https://www.scopus.com/pages/publications/0023759322#tab=citedBy

U2 - 10.1126/science.3413482

DO - 10.1126/science.3413482

M3 - Article

C2 - 3413482

AN - SCOPUS:0023759322

SN - 0036-8075

VL - 241

SP - 1188

EP - 1191

JO - Science

JF - Science

IS - 4870

ER -

Induction of an antibody that catalyzes the hydrolysis of an amide bond

Abstract

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