Induction of protective immunity against influenza A/Jiangxi-Donghu/346/2013 (H10N8) in mice

Li Fang Kuah, Lay Hoon Tang, Troy Sutton, Jie Hui Lim, Wan Ling Sin, Elaine Lamirande, Kanta Subbarao, Yuk Fai Lau

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Human infections with A/Jiangxi-Donghu/346/2013 (H10N8) virus have raised concerns about its pandemic potential. In order to develop a vaccine against this virus, the immunogenicity of its haemagglutinin protein was evaluated in mice. Using both whole-virion and recombinant subunit protein vaccines, we showed that two doses of either vaccine elicited neutralizing antibody responses. The protective efficacy of the vaccine-induced responses was assessed using a reverse-genetics-derived H10 reassortant virus on the A/Puerto Rico/8/34 (H1N1) backbone. The reassortant virus replicated efficiently in the respiratory tract of unvaccinated mice whereas vaccinated mice were completely protected from challenge, with no detectable viral load in the lower respiratory tract. Finally, the serum neutralizing antibody responses elicited by the H10 vaccines also exhibited cross-neutralizing activity against three heterologous wild-type H10 viruses. Collectively, these findings demonstrate that different vaccine platforms presenting the H10 haemagglutinin protein induce protective immunity.

Original languageEnglish (US)
Article number000683
Pages (from-to)155-165
Number of pages11
JournalJournal of General Virology
Issue number2
StatePublished - Feb 2017

All Science Journal Classification (ASJC) codes

  • Virology


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