TY - JOUR
T1 - Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells
AU - Vichchathorn, Prachya
AU - Jenwithisuk, Rachaneeporn
AU - Leelaudomlipi, Surasak
AU - Tungpradabkul, Sumalee
AU - Hongeng, Suradej
AU - Cui, Liwang
AU - Sattabongkot, Jetsumon
AU - Udomsangpetch, Rachanee
N1 - Funding Information:
The authors wish to thank all staff at Malaria Clinic in Mae Sot, Tak province, and N. Nongngork, V. Phunkitchar, K. Laptaveeshoke, J. Opasnawakul, N. Trongnipatt and K. Jangpatarapongsa for technical assistance. PV was supported by the Medical Scholar Program, Mahidol University. This work was supported by the Fogarty International Center (D43 TW006571) and the malaria vaccine program, U.S. Army Medical Research and Materiel Command.
PY - 2006/9
Y1 - 2006/9
N2 - Due to chronic morbidity, the risk of increasing drug resistance and the existence of the hypnozoite stage in Plasmodium vivax malaria, there is a need to find out how hosts develop immunity to compromise the malaria parasites. Here we focused on an in vitro model for immunotherapy and vaccine development. Immunosuppressive mechanisms in malaria include inhibition of T cell response and suppression of dendritic cell function. Using in vitro activation of lymphocytes by malaria antigen-pulsed dendritic cells could overcome the limitation of antigen presentation during acute infections. Here we showed that the sporozoite-pulsed dendritic cell could elicit cytotoxicity against liver stage of P. vivax. Analysis using immunophenotypic markers showed maturation of the dendritic cells and stimulation of cytotoxic T cells. Functional assay of the in vitro-activated cytotoxic T cells showed enhancement of specific killing of the P. vivax exoerythrocytic stages within infected hepatocytes. This model may be useful for vaccine development against human malaria.
AB - Due to chronic morbidity, the risk of increasing drug resistance and the existence of the hypnozoite stage in Plasmodium vivax malaria, there is a need to find out how hosts develop immunity to compromise the malaria parasites. Here we focused on an in vitro model for immunotherapy and vaccine development. Immunosuppressive mechanisms in malaria include inhibition of T cell response and suppression of dendritic cell function. Using in vitro activation of lymphocytes by malaria antigen-pulsed dendritic cells could overcome the limitation of antigen presentation during acute infections. Here we showed that the sporozoite-pulsed dendritic cell could elicit cytotoxicity against liver stage of P. vivax. Analysis using immunophenotypic markers showed maturation of the dendritic cells and stimulation of cytotoxic T cells. Functional assay of the in vitro-activated cytotoxic T cells showed enhancement of specific killing of the P. vivax exoerythrocytic stages within infected hepatocytes. This model may be useful for vaccine development against human malaria.
UR - http://www.scopus.com/inward/record.url?scp=33745887751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745887751&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2006.04.001
DO - 10.1016/j.parint.2006.04.001
M3 - Article
C2 - 16793328
AN - SCOPUS:33745887751
SN - 1383-5769
VL - 55
SP - 187
EP - 193
JO - Parasitology International
JF - Parasitology International
IS - 3
ER -
