TY - JOUR
T1 - Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells
AU - Takeda, Shuso
AU - Harada, Mari
AU - Su, Shengzhong
AU - Okajima, Shunsuke
AU - Miyoshi, Hiroko
AU - Yoshida, Kazutaka
AU - Nishimura, Hajime
AU - Okamoto, Yoshiko
AU - Amamoto, Toshiaki
AU - Watanabe, Kazuhito
AU - Omiecinski, Curtis J.
AU - Aramaki, Hironori
PY - 2013
Y1 - 2013
N2 - To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.
AB - To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.
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U2 - 10.2131/jts.38.305
DO - 10.2131/jts.38.305
M3 - Article
C2 - 23535410
AN - SCOPUS:84875487849
SN - 0388-1350
VL - 38
SP - 305
EP - 308
JO - Journal of Toxicological Sciences
JF - Journal of Toxicological Sciences
IS - 2
ER -
