Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells

Shuso Takeda; Mari Harada; Shengzhong Su; Shunsuke Okajima; Hiroko Miyoshi; Kazutaka Yoshida; Hajime Nishimura; Yoshiko Okamoto; Toshiaki Amamoto; Kazuhito Watanabe; Curtis J. Omiecinski; Hironori Aramaki

doi:10.2131/jts.38.305

Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ⁹-tetrahydrocannabinol in human breast cancer cells

Shuso Takeda
, Mari Harada
, Shengzhong Su
, Shunsuke Okajima
, Hiroko Miyoshi
, Kazutaka Yoshida
, Hajime Nishimura
, Yoshiko Okamoto
, Toshiaki Amamoto
, Kazuhito Watanabe
, Curtis J. Omiecinski
, Hironori Aramaki

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

To investigate gene(s) being regulated by Δ⁹-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ⁹-THC for 48 hr at an IC₅₀ concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ⁹-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ⁹-THC-regulated gene, and that Δ⁹-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.

Original language	English (US)
Pages (from-to)	305-308
Number of pages	4
Journal	Journal of Toxicological Sciences
Volume	38
Issue number	2
DOIs	https://doi.org/10.2131/jts.38.305
State	Published - 2013

All Science Journal Classification (ASJC) codes

Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2131/jts.38.305

Cite this

@article{06f3f0c4831643c8bbe48689e0c29247,

title = "Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells",

abstract = "To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.",

author = "Shuso Takeda and Mari Harada and Shengzhong Su and Shunsuke Okajima and Hiroko Miyoshi and Kazutaka Yoshida and Hajime Nishimura and Yoshiko Okamoto and Toshiaki Amamoto and Kazuhito Watanabe and Omiecinski, \{Curtis J.\} and Hironori Aramaki",

year = "2013",

doi = "10.2131/jts.38.305",

language = "English (US)",

volume = "38",

pages = "305--308",

journal = "Journal of Toxicological Sciences",

issn = "0388-1350",

publisher = "Japanese Society of Toxicology",

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TY - JOUR

T1 - Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells

AU - Takeda, Shuso

AU - Harada, Mari

AU - Su, Shengzhong

AU - Okajima, Shunsuke

AU - Miyoshi, Hiroko

AU - Yoshida, Kazutaka

AU - Nishimura, Hajime

AU - Okamoto, Yoshiko

AU - Amamoto, Toshiaki

AU - Watanabe, Kazuhito

AU - Omiecinski, Curtis J.

AU - Aramaki, Hironori

PY - 2013

Y1 - 2013

N2 - To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.

AB - To investigate gene(s) being regulated by Δ9-tetrahydrocannabinol (Δ9-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Δ9-THC for 48 hr at an IC50 concentration of approximately 25 μM. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red O staining after Δ9-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Δ9-THC-regulated gene, and that Δ9-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.

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