TY - JOUR
T1 - Inhibition of hemagglutination activity of influenza a viruses by SP-A1 and SP-A2 variants expressed in CHO cells
AU - Mikerov, Anatoly N.
AU - White, Mitch
AU - Hartshorn, Kevan
AU - Wang, Guirong
AU - Floros, Joanna
N1 - Funding Information:
Acknowledgments This work was supported by the National Institute of Environmental Health Sciences (RO1 ES009882-04A1) and HL69031 (KLH). The authors thank Dr Martin van Eijk for initial pilot experiments with glycan blots, and Karen Chorney for technical assistance.
PY - 2008/3
Y1 - 2008/3
N2 - Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed α(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to α(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to α(2,3)-linked sialic acids (like avian strains).
AB - Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed α(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to α(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to α(2,3)-linked sialic acids (like avian strains).
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U2 - 10.1007/s00430-007-0051-4
DO - 10.1007/s00430-007-0051-4
M3 - Article
C2 - 17520282
AN - SCOPUS:36348946320
SN - 0300-8584
VL - 197
SP - 9
EP - 12
JO - Medical Microbiology and Immunology
JF - Medical Microbiology and Immunology
IS - 1
ER -