Inhibition of HOS expression and activities by Wnt pathway

Vladimir S. Spiegelman; Weigang Tang; Masaru Katoh; Thomas J. Slaga; Serge Y. Fuchs

doi:10.1038/sj.onc.1205132

Inhibition of HOS expression and activities by Wnt pathway

Vladimir S. Spiegelman, Weigang Tang, Masaru Katoh, Thomas J. Slaga, Serge Y. Fuchs

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

βTrCP and HOS are closely related F-box proteins, which play key roles in ubiquitination and degradation of β-catenin and IκB through associating with those phosphorylated substrates and recruiting SCF E3 ubiquitin ligase. Here we report that activation of Wnt/β-catenin signal transduction pathway elevates βTrCP levels but inhibits expression of HOS in 293T cells. Similar disparity is likely to exist in human colorectal tumors. In the NIH3T3 cells, which express HOS, but not βTrCP, Wnt/β-catenin signaling leads to inhibition of HOS promoter activity and NF-κB-driven transcription as well as to stabilization of β-catenin. These results indicate that expression and activities of HOS are negatively regulated by Wnt/β-catenin pathway.

Original language	English (US)
Pages (from-to)	856-860
Number of pages	5
Journal	Oncogene
Volume	21
Issue number	5
DOIs	https://doi.org/10.1038/sj.onc.1205132
State	Published - Jan 24 2002

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Cancer Research

UN SDGs

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10.1038/sj.onc.1205132

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title = "Inhibition of HOS expression and activities by Wnt pathway",

abstract = "βTrCP and HOS are closely related F-box proteins, which play key roles in ubiquitination and degradation of β-catenin and IκB through associating with those phosphorylated substrates and recruiting SCF E3 ubiquitin ligase. Here we report that activation of Wnt/β-catenin signal transduction pathway elevates βTrCP levels but inhibits expression of HOS in 293T cells. Similar disparity is likely to exist in human colorectal tumors. In the NIH3T3 cells, which express HOS, but not βTrCP, Wnt/β-catenin signaling leads to inhibition of HOS promoter activity and NF-κB-driven transcription as well as to stabilization of β-catenin. These results indicate that expression and activities of HOS are negatively regulated by Wnt/β-catenin pathway.",

author = "Spiegelman, {Vladimir S.} and Weigang Tang and Masaru Katoh and Slaga, {Thomas J.} and Fuchs, {Serge Y.}",

note = "Funding Information: We appreciate the comments of unknown reviewers, which helped us to improve this manuscript. We thank P Stavropoulos for constructing the HOS promoter-driven luciferase reporter. We are grateful to Dr F Luca for critical suggestions. We also thank Drs S Aaronson, B Vogelstein, J Manfredi and P Polakis for reagents. The work was supported in part by NIH grants CA 92900 (to SY Fuchs) and CA 76262 (to TJ Slaga).",

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doi = "10.1038/sj.onc.1205132",

language = "English (US)",

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T1 - Inhibition of HOS expression and activities by Wnt pathway

AU - Spiegelman, Vladimir S.

AU - Tang, Weigang

AU - Katoh, Masaru

AU - Slaga, Thomas J.

AU - Fuchs, Serge Y.

N1 - Funding Information: We appreciate the comments of unknown reviewers, which helped us to improve this manuscript. We thank P Stavropoulos for constructing the HOS promoter-driven luciferase reporter. We are grateful to Dr F Luca for critical suggestions. We also thank Drs S Aaronson, B Vogelstein, J Manfredi and P Polakis for reagents. The work was supported in part by NIH grants CA 92900 (to SY Fuchs) and CA 76262 (to TJ Slaga).

PY - 2002/1/24

Y1 - 2002/1/24

N2 - βTrCP and HOS are closely related F-box proteins, which play key roles in ubiquitination and degradation of β-catenin and IκB through associating with those phosphorylated substrates and recruiting SCF E3 ubiquitin ligase. Here we report that activation of Wnt/β-catenin signal transduction pathway elevates βTrCP levels but inhibits expression of HOS in 293T cells. Similar disparity is likely to exist in human colorectal tumors. In the NIH3T3 cells, which express HOS, but not βTrCP, Wnt/β-catenin signaling leads to inhibition of HOS promoter activity and NF-κB-driven transcription as well as to stabilization of β-catenin. These results indicate that expression and activities of HOS are negatively regulated by Wnt/β-catenin pathway.

AB - βTrCP and HOS are closely related F-box proteins, which play key roles in ubiquitination and degradation of β-catenin and IκB through associating with those phosphorylated substrates and recruiting SCF E3 ubiquitin ligase. Here we report that activation of Wnt/β-catenin signal transduction pathway elevates βTrCP levels but inhibits expression of HOS in 293T cells. Similar disparity is likely to exist in human colorectal tumors. In the NIH3T3 cells, which express HOS, but not βTrCP, Wnt/β-catenin signaling leads to inhibition of HOS promoter activity and NF-κB-driven transcription as well as to stabilization of β-catenin. These results indicate that expression and activities of HOS are negatively regulated by Wnt/β-catenin pathway.

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Inhibition of HOS expression and activities by Wnt pathway

Abstract

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