Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Reyad A. Elbarbary; Hiroaki Takaku; Masato Tamura; Masayuki Nashimoto

doi:10.1016/j.bbrc.2008.12.173

Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Reyad A. Elbarbary, Hiroaki Takaku, Masato Tamura, Masayuki Nashimoto

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Abstract

Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase Z^L. To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogenous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely.

Original language	English (US)
Pages (from-to)	924-927
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	379
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.12.173
State	Published - Feb 20 2009

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2008.12.173

Cite this

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title = "Inhibition of vascular endothelial growth factor expression by TRUE gene silencing",

abstract = "Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase ZL. To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogenous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely.",

author = "Elbarbary, {Reyad A.} and Hiroaki Takaku and Masato Tamura and Masayuki Nashimoto",

note = "Funding Information: We thank Dr. T. Hashiguchi for providing us the plasmid pCI/hVEGF-A. This work was supported in part by a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports and Technology of Japan.",

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T1 - Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

AU - Elbarbary, Reyad A.

AU - Takaku, Hiroaki

AU - Tamura, Masato

AU - Nashimoto, Masayuki

N1 - Funding Information: We thank Dr. T. Hashiguchi for providing us the plasmid pCI/hVEGF-A. This work was supported in part by a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports and Technology of Japan.

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N2 - Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase ZL. To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogenous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely.

AB - Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase ZL. To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogenous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely.

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Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

Abstract

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