Inoculation and tissue distribution in pilot-scale plant root culture bioreactors

Divakar Ramakrishnan, John Salim, Wayne R. Curtis

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Inoculation of large-scale plant root culture reactors can be carried out by briefly homogenizing bulk root tissue, followed by aseptic transfer as a slurry to the reactor. Uniform root distribution can be achieved in bioreactors by entrapment of the growing root inoculum onto process packing elements (e.g. distillation packings), randomly distributed within the reactor, in a bubble column operation. These two techniques have been successfully used to inoculate a 14 L pilot-scale reactor which is subsequently operated as a trickle bed reactor.

Original languageEnglish (US)
Pages (from-to)639-644
Number of pages6
JournalBiotechnology Techniques
Issue number9
StatePublished - Sep 1994

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology


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