Inorganic nitrate supplementation and blood flow restricted exercise tolerance in post-menopausal women

David N. Proctor, Kristina A. Neely, Swapan Mookerjee, Jacqueline Tucker, Yasina B. Somani, Michael Flanagan, Daniel B. Kim-Shapiro, Swati Basu, Matthew D. Muller, Danielle Jin-Kwang Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Exercise tolerance appears to benefit most from dietary nitrate (NO3) supplementation when muscle oxygen (O2) availability is low. Using a double-blind, randomized cross-over design, we tested the hypothesis that acute NO3 supplementation would improve blood flow restricted exercise duration in post-menopausal women, a population with reduced endogenous nitric oxide bioavailability. Thirteen women (57–76 yr) performed rhythmic isometric handgrip contractions (10% MVC, 30 per min) during progressive forearm blood flow restriction (upper arm cuff gradually inflated 20 mmHg each min) on three study visits, with 7–10 days between visits. Approximately one week following the first (familiarization) visit, participants consumed 140 ml of NO3 concentrated (9.7 mmol, 0.6 gm NO3) or NO3depleted beetroot juice (placebo) on separate days (≥7 days apart), with handgrip exercise beginning 100 min post-consumption. Handgrip force recordings were analyzed to determine if NO3 supplementation enhanced force development as blood flow restriction progressed. Nitrate supplementation increased plasma NO3 (16.2-fold) and NO2 (4.2-fold) and time to volitional fatigue (61.8 ± 56.5 s longer duration vs. placebo visit; p = 0.03). Nitrate supplementation increased the rate of force development as forearm muscle ischemia progressed (p = 0.023 between 50 and 75% of time to fatigue) with non-significant effects thereafter (p = 0.052). No effects of nitrate supplementation were observed for mean duration of contraction or relaxation rates (all p > 0.150). These results suggest that acute NO3 supplementation prolongs time-to-fatigue and speeds grip force development during progressive forearm muscle ischemia in postmenopausal women.

Original languageEnglish (US)
Pages (from-to)26-34
Number of pages9
JournalNitric Oxide - Biology and Chemistry
StatePublished - May 1 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research


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