Insights into GATA-1-Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis

Ming Yu, Laura Riva, Huafeng Xie, Yocheved Schindler, Tyler B. Moran, Yong Cheng, Duonan Yu, Ross Hardison, Mitchell J. Weiss, Stuart H. Orkin, Bradley E. Bernstein, Ernest Fraenkel, Alan B. Cantor

Research output: Contribution to journalArticlepeer-review

260 Scopus citations

Abstract

The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1-induced gene expression changes. Bound and differentially expressed genes contain a greater number of GATA-binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to the transcriptional start site versus nondifferentially expressed genes. Moreover, we show that the transcription factor Zbtb7a occupies GATA-1-bound regions of some direct GATA-1 target genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus repression, and that polycomb repressive complex 2 (PRC2) is involved in epigenetic silencing of a subset of GATA-1-repressed genes. These data provide insights into GATA-1-mediated gene regulation in vivo.

Original languageEnglish (US)
Pages (from-to)682-695
Number of pages14
JournalMolecular cell
Volume36
Issue number4
DOIs
StatePublished - Nov 25 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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