Integrated PCR and thermal melt analysis on a microfluidic chip

Deborah Boles; Michele Stone; Esther Huang; Ming Wu; Roger Dettloff; Aaron Rulison; Javier Farinas; Ivor Knight

Integrated PCR and thermal melt analysis on a microfluidic chip

Deborah Boles, Michele Stone, Esther Huang, Ming Wu, Roger Dettloff, Aaron Rulison, Javier Farinas, Ivor Knight

School of Science (Behrend)

Research output: Contribution to conference › Paper › peer-review

Abstract

We have modified and tested the microfluidic LabChip® system [1] to integrate thermal melt analysis and PCR in a continuous flow format utilizing reaction volumes of several nanoliters. Results demonstrate high resolution thermal melt analysis at ramp rates up to 1°C/s. To our knowledge, this is the first demonstration of a continuous flow system integrating PCR and thermal melt analysis.

Original language	English (US)
Pages	1444-1446
Number of pages	3
State	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Integrated PCR and thermal melt analysis on a microfluidic chip",

abstract = "We have modified and tested the microfluidic LabChip{\textregistered} system [1] to integrate thermal melt analysis and PCR in a continuous flow format utilizing reaction volumes of several nanoliters. Results demonstrate high resolution thermal melt analysis at ramp rates up to 1°C/s. To our knowledge, this is the first demonstration of a continuous flow system integrating PCR and thermal melt analysis.",

author = "Deborah Boles and Michele Stone and Esther Huang and Ming Wu and Roger Dettloff and Aaron Rulison and Javier Farinas and Ivor Knight",

year = "2006",

language = "English (US)",

pages = "1444--1446",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Integrated PCR and thermal melt analysis on a microfluidic chip

AU - Boles, Deborah

AU - Stone, Michele

AU - Huang, Esther

AU - Wu, Ming

AU - Dettloff, Roger

AU - Rulison, Aaron

AU - Farinas, Javier

AU - Knight, Ivor

PY - 2006

Y1 - 2006

N2 - We have modified and tested the microfluidic LabChip® system [1] to integrate thermal melt analysis and PCR in a continuous flow format utilizing reaction volumes of several nanoliters. Results demonstrate high resolution thermal melt analysis at ramp rates up to 1°C/s. To our knowledge, this is the first demonstration of a continuous flow system integrating PCR and thermal melt analysis.

AB - We have modified and tested the microfluidic LabChip® system [1] to integrate thermal melt analysis and PCR in a continuous flow format utilizing reaction volumes of several nanoliters. Results demonstrate high resolution thermal melt analysis at ramp rates up to 1°C/s. To our knowledge, this is the first demonstration of a continuous flow system integrating PCR and thermal melt analysis.

UR - http://www.scopus.com/inward/record.url?scp=84902477724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902477724&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902477724

SP - 1444

EP - 1446

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Integrated PCR and thermal melt analysis on a microfluidic chip

Abstract

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All Science Journal Classification (ASJC) codes

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