TY - JOUR
T1 - Interaction with dopamine D2 receptor enhances expression of transient receptor potential channel 1 at the cell surface
AU - Hannan, Meredith A.
AU - Kabbani, Nadine
AU - Paspalas, Constantinos D.
AU - Levenson, Robert
N1 - Funding Information:
We are grateful to Drs. Victor Canfield, Blaise Peterson, and Jamie Weiss for their helpful comments on the manuscript. This work was supported by an NIH Conte Center grant (MH 068789).
PY - 2008/4
Y1 - 2008/4
N2 - Receptor signaling is mediated by direct protein interaction with various types of cytoskeletal, adapter, effector, and additional receptor molecules. In brain tissue and in cultured neurons, activation of dopamine D2 receptors (D2Rs) has been found to impact cellular calcium signaling. Using a yeast two-hybrid approach, we have uncovered a direct physical interaction between the D2R and the transient receptor potential channel (TRPC) subtypes 1, 4 and 5. The TRPC/D2R interaction was further validated by GST-pulldown assays and coimmunoprecipitation from mammalian brain. Ultrastructural analysis of TRPC1 and D2R expression indicates colocalization of the two proteins within the cell body and dendrites of cortical neurons. In cultured cells, expression of D2Rs was found to increase expression of TRPC1 at the cell surface by 50%. These findings shed new light on the constituents of the D2R signalplex, and support the involvement of D2Rs in cellular calcium signaling pathways via a novel link to TRPC channels.
AB - Receptor signaling is mediated by direct protein interaction with various types of cytoskeletal, adapter, effector, and additional receptor molecules. In brain tissue and in cultured neurons, activation of dopamine D2 receptors (D2Rs) has been found to impact cellular calcium signaling. Using a yeast two-hybrid approach, we have uncovered a direct physical interaction between the D2R and the transient receptor potential channel (TRPC) subtypes 1, 4 and 5. The TRPC/D2R interaction was further validated by GST-pulldown assays and coimmunoprecipitation from mammalian brain. Ultrastructural analysis of TRPC1 and D2R expression indicates colocalization of the two proteins within the cell body and dendrites of cortical neurons. In cultured cells, expression of D2Rs was found to increase expression of TRPC1 at the cell surface by 50%. These findings shed new light on the constituents of the D2R signalplex, and support the involvement of D2Rs in cellular calcium signaling pathways via a novel link to TRPC channels.
UR - http://www.scopus.com/inward/record.url?scp=40949124283&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40949124283&partnerID=8YFLogxK
U2 - 10.1016/j.bbamem.2008.01.011
DO - 10.1016/j.bbamem.2008.01.011
M3 - Article
C2 - 18261457
AN - SCOPUS:40949124283
SN - 0005-2736
VL - 1778
SP - 974
EP - 982
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 4
ER -