Interactions of C. frondosa-derived inhibitory peptides against angiotensin I-converting enzyme (ACE), α-amylase and lipase

Yi Zhang, Shudong He, Xin Rui, Benjamin K. Simpson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The study illustrates the molecular mechanisms by which marine-derived peptides exhibited different structures and inhibition functions to concurrently inhibit multiple enzymes involved in chronic diseases. Peptides (2 mg/mL) exhibited inhibition against angiotensin-converting enzyme (ACE, inhibition of 52.2–78.8%), pancreatic α-amylase (16.3–27.2%) and lipase (5.3–17.0%). Further in silico analyses on physiochemistry, bioactivity, safety and interaction energy with target enzymes indicated that one peptide could inhibit multiple enzymes. Peptide FENLLEELK potent in inhibiting both ACE and α-amylase showed different mechanisms: it had ordered extended structure in ACE active pocket with conventional H-bond towards Arg522 which is the ligand for activator Cl-, while the peptide folded into compact “lariat” conformation within α-amylase active site and the K residue in peptide formed intensive H-bonds and electrostatic interactions with catalytic triad Asp197 − Asp300 − Glu233. Another peptide APFPLR showed different poses in inhibiting ACE, α-amylase and lipase, and it formed direct interactions to lipase catalytic residues Phe77 & His263.

Original languageEnglish (US)
Article number130695
JournalFood Chemistry
Volume367
DOIs
StatePublished - Jan 15 2022

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

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